L-Glutamine (200 mM), 20 x 100 mL - FAQs

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3 个常见问题解答

为何我的200 mM L-谷氨酰胺在化冻后出现沉淀？

浓储存液中的L-谷氨酰胺在低温下易于发生沉淀。在37°C水浴中短暂加热储存液并辅以温和的摇动，就可溶解沉淀。在沉淀完全溶解之前不要使用本品。

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.