碳酸氢钠 7.5% 溶液

碳酸氢钠是在存在 4–10% 二氧化碳的条件下维持细胞培养基 pH 值的常用缓冲液。除了进行缓冲，碳酸氢钠还具有某些营养优势，同时很少表现出任何细胞毒性。Gibco™ 碳酸氢钠 (7.5%) 溶液配制成了解更多信息

货号	数量
25080094	100 mL

货号 25080094

价格（CNY)

285.00

添加至购物车

100 mL

价格（CNY)

285.00

添加至购物车

碳酸氢钠是在存在 4–10% 二氧化碳的条件下维持细胞培养基 pH 值的常用缓冲液。除了进行缓冲，碳酸氢钠还具有某些营养优势，同时很少表现出任何细胞毒性。Gibco™ 碳酸氢钠 (7.5%) 溶液配制成 75 g/L 水，是在复溶过程中用于补充干粉培养基的出色选择。培养基中使用的最终碳酸氢钠浓度取决于培养基配方和孵育器中所用的二氧化碳浓度。

产品用途
仅供研究使用。不可用于诊断程序。

双现场机制 cGMP 生产和质量体系
Gibco™ 碳酸氢钠溶液在位于纽约格兰德岛上符合 cGMP 要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商，且通过ISO 13485标准认证。为确保供应链的稳定，我们同时提供由我们的苏格兰工厂生产的相同 Gibco™ 碳酸氢钠溶液产品 (25080-060)。后者亦是在FDA登记的医疗仪器生产商，且符合ISO 13485标准。

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞

最大浓度0.075

数量100 mL

有效期18 个月

运输条件室温

产品类型碳酸氢钠

无菌无菌过滤

pH8.3

Unit SizeEach

内容与储存

储存条件：15 – 30°C
运输条件：环境
有效期：自生产之日起18个月

常见问题解答 (FAQ)

Based on the Sodium bicarbonate levels in the medium, what CO2 % is recommended?

If the media formulation contains:

NaHCO3 (g/L) < 1.5, it needs CO2 at 4%;
NaHCO3 (g/L) 1.5 – 2.2, it needs CO2 at 5%;
NaHCO3 (g/L) 2.2 - 3.4, it needs CO2 at 7%;
NaHCO3 (g/L) > 3.5, it needs CO2 at 10% .

*There are some exceptions. Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7 g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. This also depends on the cell type. Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do high or low levels of sodium bicarbonate affect the pH of my tissue culture media?

Sodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator*.

If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH.

If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.

*There are some exceptions. For instance, Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. this also depends on the cell type, Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.