Samco™ Narrow Stem Transfer Pipettes, 15.0 mL Sedi-Pet Transfer Pipets, Narrow Stem, Large bulb, 15.0 mL Sedi-Pet™ Transfer Pipets, Narrow Stem, Large bulb (250/Pack, 2500/Case) - Citations
Samco™ Narrow Stem Transfer Pipettes, 15.0 mL Sedi-Pet Transfer Pipets, Narrow Stem, Large bulb, 15.0 mL Sedi-Pet™ Transfer Pipets, Narrow Stem, Large bulb (250/Pack, 2500/Case) - Citations
Characterization of the SIM-A9 cell line as a model of activated microglia in the context of neuropathic pain.
AuthorsDave KM, Ali L, Manickam DS
JournalPLoS One
PubMed ID32287325
'Resident microglia of the central nervous system are being increasingly recognized as key players in diseases such as neuropathic pain. Biochemical and behavioral studies in neuropathic pain rodent models have documented compelling evidence of the critical role of ATP mediated-P2X4R-brain-derived neurotrophic factor (BDNF) signaling pathway in the initiation and maintenance ... More
Expression of the neonatal Fc-receptor in placental-fetal endothelium and in cells of the placental immune system.
AuthorsKiskova T, Mytsko Y, Schepelmann M, Helmer H, Fuchs R, Miedl H, Wadsack C, Ellinger I
JournalPlacenta
PubMed ID30955709
'Starting from the second trimester of pregnancy, passive immunity is provided to the human fetus by transplacental transfer of maternal IgG. IgG transfer depends on the neonatal Fc receptor, FcRn. While FcRn localization in the placental syncytiotrophoblast (STB) has been demonstrated unequivocally, FcRn expression in placental-fetal endothelial cells (pFECs), which ... More
Sleep-restriction Inhibits Neurogenesis Through Decreasing the Infiltration of CD169
AuthorsYao H, Zhang Y, Xie B, Shang Y, Yuan S, Zhang J
JournalNeuroscience
PubMed ID32081723
'Chronic sleep-restriction (SR) is shown to be correlated with neurodevelopmental disorders. However, the effects of SR during stroke recovery on neurorepair remain unclear. In this study, mice were subjected to 60?min of cerebral ischemia followed by reperfusion. The SR protocol was accomplished by depriving mice of sleep for 20?h/day for ... More
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
AuthorsTian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M
JournalNeuron
PubMed ID31422865
CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in human neurons derived from induced pluripotent stem cells (iPSCs). We ... More
The WNT/ß-catenin signaling inhibitor XAV939 enhances the elimination of LNCaP and PC-3 prostate cancer cells by prostate cancer patient lymphocytes in vitro.
AuthorsStakheev D, Taborska P, Strizova Z, Podrazil M, Bartunkova J, Smrz D
JournalSci Rep
PubMed ID30886380
Upregulated Wnt/ß-catenin signaling is associated with increased cancer cell resistance and cancer cell-elicited immunosuppression. In non-neoplastic immune cells, upregulated Wnt/ß-catenin is, however, associated with either immunosuppression or immunostimulation. Therefore, it is difficult to predict the therapeutic impact inhibitors of Wnt/ß-catenin signaling will have when combined with cancer immunotherapy. Here, we ... More
Cellular cytotoxicity is a form of immunogenic cell death.
AuthorsMinute L, Teijeira A, Sanchez-Paulete AR, Ochoa MC, Alvarez M, Otano I, Etxeberrria I, Bolaños E, Azpilikueta A, Garasa S, Casares N, Luis Perez Gracia J, Rodriguez-Ruiz ME, Berraondo P, Melero I
JournalJ Immunother Cancer
PubMed ID32217765
The immune response to cancer is often conceptualized with the cancer immunity cycle. An essential step in this interpretation is that antigens released by dying tumors are presented by dendritic cells to naive or memory T cells in the tumor-draining lymph nodes. Whether tumor cell death resulting from cytotoxicity, as ... More
N-terminal variant Asp14Asn of the human p70 S6 Kinase 1 enhances translational signaling causing different effects in developing and mature neuronal cells.
AuthorsVenkatasubramani JP, Subramanyam P, Pal R, Reddy BK, Srinivasan DJ, Chattarji S, Iossifov I, Klann E, Bhattacharya A
JournalNeurobiol Learn Mem
PubMed ID32147585
The ribosomal p70 S6 Kinase 1 (S6K1) has been implicated in the etiology of complex neurological diseases including autism, depression and dementia. Though no major gene disruption has been reported in humans in RPS6KB1, single nucleotide variants (SNVs) causing missense mutations have been identified, which have not been assessed for ... More
Routine Optical Clearing of 3D-Cell Cultures: Simplicity Forward.
AuthorsNürnberg E, Vitacolonna M, Klicks J, von Molitor E, Cesetti T, Keller F, Bruch R, Ertongur-Fauth T, Riedel K, Scholz P, Lau T, Schneider R, Meier J, Hafner M, Rudolf R
JournalFront Mol Biosci
PubMed ID32154265
Three-dimensional cell cultures, such as spheroids and organoids, serve as increasingly important models in fundamental and applied research and start to be used for drug screening purposes. Optical tissue clearing procedures are employed to enhance visualization of fluorescence-stained organs, tissues, and three-dimensional cell cultures. To get a more systematic overview ... More
AuthorsLi D, Matta B, Song S, Nelson V, Diggins K, Simpfendorfer KR, Gregersen PK, Linsley P, Barnes BJ
JournalJCI Insight
PubMed ID31877114
Genetic variants within or near the interferon regulatory factor 5 (IRF5) locus associate with systemic lupus erythematosus (SLE) across ancestral groups. The major IRF5-SLE risk haplotype is common across populations, yet immune functions for the risk haplotype are undefined. We characterized the global immune phenotype of healthy donors homozygous for ... More
Apoptotic Cell Exclusion and Bias-Free Single-Cell Selection Are Important Quality Control Requirements for Successful Single-Cell Sequencing Applications.
AuthorsOrdoñez-Rueda D, Baying B, Pavlinic D, Alessandri L, Yeboah Y, Landry JJM, Calogero R, Benes V, Paulsen M
JournalCytometry A
PubMed ID31603610
Single-cell sequencing experiments are a new mainstay in biology and have been advancing science especially in the biomedical field. The high pressure to integrate the technology into daily laboratory live requires solid knowledge with respect to potential limitations and precautions to be taken care of before applying it to complex ... More