Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.
Authors Crowe J S; Cooper H J; Smith M A; Sims M J; Parker D; Gewert D;
JournalNucleic Acids Res
PubMed ID2011503
Not available
Regulation of calreticulin expression during induction of differentiation in human myeloid cells. Evidence for remodeling of the endoplasmic reticulum.
Authors Clark Robert A; Li Sen-Lin; Pearson Doran W; Leidal Kevin G; Clark Joshua R; Denning Gerene M; Reddick Robert; Krause Karl-Heinz; Valente Anthony J;
JournalJ Biol Chem
PubMed ID12065601
Induction of differentiation of HL-60 human myeloid cells profoundly affected expression of calreticulin, a Ca(2+)-binding endoplasmic reticulum chaperone. Induction with Me(2)SO or retinoic acid reduced levels of calreticulin protein by approximately 60% within 4 days. Pulse-chase studies indicated that labeled calreticulin decayed at similar rates in differentiated and undifferentiated cells ... More