How long and at what temperature can the Pierce Traut's Reagent (2-iminothiolane) (Cat. No. 26101) be stored in acidic solution devoid of primary amines?
We do not recommend storing the Traut’s Reagent in solution. This reagent is more stable in solution than others crosslinkers because hydrolysis takes place at a slower rate. Yet, its hydrolysis will inevitably occur with time if it is in solution. Therefore, to ensure a better crosslinking efficiency, we recommend preparing a fresh solution each time and storing the Traut's reagent as a powder at 4°C. Unfortunately, we do not have stability data of this reagent in a solution.
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Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.