Pierce™ 琼脂糖 ChIP 试剂盒
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Pierce™ 琼脂糖 ChIP 试剂盒
Thermo Scientific™

Pierce™ 琼脂糖 ChIP 试剂盒

Thermo Scientific Pierce 琼脂糖 ChIP 试剂盒提供了一整套试剂以及简单、快速且可重现的方案,可进行染色质免疫沉淀 (ChIP) 检测。琼脂糖了解更多信息
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货号数量
2615630 次反应试剂盒
货号 26156
价格(CNY)
6,117.00
飞享价
Ends: 31-Dec-2025
9,551.00
共减 3,434.00 (36%)
Each
添加至购物车
数量:
30 次反应试剂盒
请求批量或定制报价
价格(CNY)
6,117.00
飞享价
Ends: 31-Dec-2025
9,551.00
共减 3,434.00 (36%)
Each
添加至购物车
Thermo Scientific Pierce 琼脂糖 ChIP 试剂盒提供了一整套试剂以及简单、快速且可重现的方案,可进行染色质免疫沉淀 (ChIP) 检测。

琼脂糖 ChIP 试剂盒的特点:

•简单且快速的 ChIP 检测方案(< 8 小时)
•高效核分离和裂解
•简单且可重现的酶酶切
•低背景和高结合能力蛋白 A/G 琼脂糖树脂
•高度特异性的阳性对照试剂包括:RNA 聚合酶 II 抗体以及 GAPDH PCR 引物
•快速且可重现的离心柱规格
•高回收率 DNA 纯化
•可提供经验证适用于 ChIP 的抗体

Pierce 琼脂糖 ChIP 试剂盒包含足够试剂,可使用优化的方案和便利的离心柱格式采用适当对照品进行 30 次 ChIP 检测。我们也提供经验证适用于 ChIP 且具备质量保证的抗体,可与 Pierce 琼脂糖 ChIP 试剂盒一起使用。因为适用于 Western 印迹的抗体可能在 ChIP 检测中不起作用,所以我们组装了较广泛采用的经验证适用于 ChIP 的单克隆和多克隆抗体,以使从实验设置至获得结果这一过程的进展更快。

ChIP 检测的优势在于,它能够捕获活细胞中特异性蛋白-DNA 相互作用的快照,然后使用标准或定量 PCR 来定量分析相互作用。要想成功进行 ChIP 检测,需要在检测靶标基因组 DNA 序列之前执行多个关键步骤(交联、染色质制备、免疫沉淀)。单独优化 ChIP 检测方案中的每个步骤都可能非常耗时。Pierce 琼脂糖 ChIP 检测试剂盒简化了流程,确保更易获得准确且可重现的结果。

要使用 Pierce 琼脂糖 ChIP 试剂盒进行 ChIP 检测,使蛋白-DNA 复合物保持稳定,然后进行提取。通常使用甲醛实现体内交联。直接在细胞中进行交联会锁定蛋白-DNA 复合物,从而捕获这些不稳定且有时是瞬时的相互作用。为了裂解、提取和溶解交联复合物,ChIP 检测试剂盒包括 Thermo Scientific 染色质制备模块(也可单独出售,产品编号 26158)。总之,这一全套 ChIP 检测试剂盒提供了一种简单、可靠且便利的方法,该方法可用于分离染色质结合 DNA 且可在不使用杜恩斯均质仪的情况下富集目标蛋白的样品,且来自其他细胞区室的污染小于 15%。

相关产品
Pierce™ 染色质制备模块
Pierce™ ChIP 级蛋白 A/G Plus 琼脂糖
蛋白酶 K
仅供科研使用。不可用于诊断程序。
规格
结合功能>50 mg 人 IgG/mL 树脂结合容量
描述Pierce 琼脂糖 ChIP 试剂盒
产品规格试剂盒
数量30 次反应试剂盒
足够用于30 次反应
容量(公制)结合 ≥50 mg 人 IgG/mL 树脂
产品线Pierce™
类型琼脂糖 ChIP 试剂盒
Unit SizeEach
内容与储存
足够用于:30 次完整染色质 IP 检测实验• ChIP 级蛋白 A/G Plus 琼脂糖,0.65 mL(在 4°C 下储存)
• IP 稀释/洗涤缓冲液 (5X),11 mL(在 4°C 下储存)
• IP 洗涤缓冲液 3 (5X),4.5 mL(在 4°C 下储存)
• IP 洗脱缓冲液 (2X),4.5 mL(在 4°C 下储存)
• 色谱柱附件包,3 包(在室温或 4°C 下储存)
• 微量离心管 (1.5 mL),75 管(在室温或 4°C 下储存)
• DNA 纯化柱,40 柱(在室温或 4°C 下储存)
• DNA 柱结合溶液,30 mL(在室温或 4°C 下储存)
• DNA 柱洗涤液,6 mL(在室温或 4°C 下储存)
• pH 值指示器,0.8 mL(在室温或 4°C 下储存)
• DNA 柱洗脱溶液,5 mL(在室温或 4°C 下储存)
• 抗 RNA 聚合酶 II 抗体,25 μL(在 -20°C 下储存)
• 正常兔 IgG (1 mg/mL),10 μL(在 -20°C 下储存)
• ChIP 阳性对照引物(GAPDH 启动子,人特异性),100 μL(在 -20°C 下储存)
• Pierce 染色质制备模块(产品编号 26158)(在 -20°C 下储存蛋白酶 K 和微球菌核酸酶。在 4°C 下储存所有其他组分)。

常见问题解答 (FAQ)

I am having trouble with leaking columns while using the Pierce Agarose ChIP Kit (Cat. No. 26156). How can I resolve this issue?

It is common to experience leaking when using the columns, even when the plug is pushed in very tightly. We recommend wrapping the plug with parafilm to prevent leaking.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With my ChIP assay (Agarose ChIP Kit/Magnetic ChIP Kit), the qPCR signal (cycle threshold) from my positive and negative controls show no difference or are very close. Why is this?

-Verify that your specific antibody (if not using the kit-provided RNA polymerase II antibody) is validated for IP. Ideally, a ChIP validated antibody is the best, but an antibody for IP has a good chance of working in ChIP.
-Ensure that your chromatin is properly digested (see Appendix A in the manual). Too much digestion as well as too little digestion will affect the success of the ChIP reaction.
-Ensure that all the chromatin has been released from the nuclei. When following the Magnetic ChIP kit instructions, MNase digestion of 4x106 cells followed by sonication to lyse the nuclei, yields about 20-50 µg for the IP. This same sequence can be used with the Agarose ChIP Kit as well. It is recommended that you start with 2–4 x 106 cells per ChIP reaction. Once a successful ChIP has been run at this cell number, it is possible to decrease the cell amount empirically. We have seen good results using as little at 10,000 cells, but this entirely depends on the cell line, target, and antibody.
-Ensure that enough DNA was used for qPCR. Typically, 30-80 ng of DNA is a good range.

With my Agarose ChIP Kit, what can cause no or low PCR signal in the experimental IP samples?

Here are possible causes and solutions:

- Insufficient amount of sample DNA added to the PCR reaction: Add more sample DNA to the PCR reaction.
- Insufficient amount of antibody added to the IP: Add more antibody to the IP.
- Antibody did not function in an IP: Verify that the antibody is qualified for CHIP or IP applications and has been handled and stored properly.

Note: Increasing the stringency of the nuclear lysis is not recommended unless there is no signal or low signal-to-noise in the IP. Excess sample can result in high background decreasing the signal of the specific signal.

With my Agarose ChIP Kit, what can cause PCR signal of the positive and negative control IP samples to be equivalent?

Here are possible causes and solutions:

- Insufficient washing of the IP complex: Include an additional wash with Buffers 2 and 3.
- Excess chromatin or antibody added to the IP: Add less chromatin or antibody.
- PCR amplification was measured outside the linear range of amplification: Decrease the number of amplification cycles used in the PCR reaction.

With my Agarose ChIP Kit, what can cause no or low PCR signal in the positive control IP samples?

Here are possible causes and solutions:

- Insufficient chromatin amount in the IP reaction: Use at least 25 µg of chromatin for each IP.
- Insufficient antibody incubation time: Incubate antibody overnight.
- Incomplete elution from the Protein A/G agarose resin: Perform elution at 65 degrees C and increase frequency of mixing.