Pierce™ NHS 活化琼脂糖离心柱,0.2 mL
Product Image
Thermo Scientific™

Pierce™ NHS 活化琼脂糖离心柱,0.2 mL

Thermo Scientific Pierce NHS 活化琼脂糖离心柱 (0.2 mL) 含有高质量的胺反应性微珠状琼脂糖树脂,可通过伯胺快速稳定地固定蛋白、肽和其他配体。NHS了解更多信息
Have Questions?
货号数量
2619825柱
货号 26198
价格(CNY)
3,328.00
Each
添加至购物车
数量:
25柱
请求批量或定制报价
价格(CNY)
3,328.00
Each
添加至购物车
Thermo Scientific Pierce NHS 活化琼脂糖离心柱 (0.2 mL) 含有高质量的胺反应性微珠状琼脂糖树脂,可通过伯胺快速稳定地固定蛋白、肽和其他配体。

NHS 活化琼脂糖的特点:

易于使用—只需极短的手动操作时间即可在简单的一步法反应中进行固定
快速高效—对于大多数蛋白在 30 分钟内偶联超过 85%
创新性规格—干琼脂糖可浓缩样品,使其适用于固定稀释蛋白
安全—无需危险化学品(如氰基硼氢化钠、溴化氰)
通用—亲和树脂可适应柱和批次亲和色谱技术和 FPLC 应用
兼容—可与任何含伯胺的化合物配合使用
可重复使用—防渗漏化学反应意味着您可以重复使用亲和树脂
高结合容量—结合容量为 > 25 mg/mL(干树脂)

NHS 活化琼脂糖是交联 6% 微珠状琼脂糖树脂,含 N-羟基琥珀酰亚胺 (NHS) 官能团。活化树脂与伯胺反应,以形成共价固定抗体或其他蛋白的稳定酰胺键,可用于亲和纯化程序。对于复溶干树脂,Pierce NHS 活化琼脂糖的偶联容量大于 25 mg/mL。

应用:
• 快速固定山羊抗小鼠和抗兔抗体,以纯化动物或杂交瘤中产生的 IgG。
•大量固定蛋白 A,用于纯化单克隆抗体
• 固定配体,用于纯化重组蛋白

Pierce NHS 活化琼脂糖树脂使用可靠的 NHS 酯化学,且无需危险化学品用于固定。其他胺反应载体,如高碘酸盐氧化树脂,使用有毒的氰基硼氢化钠稳定至伯胺基的反应连结,并需4至6小时才能完成。传统方法(如溴化氰活化支持物)还会偶联胺;然而,这种化学反应会导致非特异性结合和已偶联配体持续缓慢渗透。与 Pierce NHS 活化琼脂糖的反应可在一小时内完成,并且产生更加稳定的键。

在 pH 值 7-9 的无胺缓冲液中进行 NHS 活化琼脂糖偶联反应。无论配合基体的分子量或pl多少,蛋白耦合效率通常都高于80%。一旦已固定一个配合基体,就可在亲和纯化程序中多次使用制备好的树脂。交联微珠状琼脂糖具有快速线性流动潜力,可用于重力流和低至中等压力应用。

其具有独特的干形式,因此无需在丙酮溶剂中储存或去除和处理丙酮溶剂。此外,干树脂适用于与稀释样品的偶联反应,因为它会随树脂膨胀而使样品浓缩,减少了起始材料的体积并产生高效配体固定。

相关产品
Pierce™ NHS 活化琼脂糖,干
Pierce™ NHS 活化琼脂糖浆液
Pierce™ NHS 活化琼脂糖离心柱,2 mL
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
适用于(应用)亲和色谱
产品规格微量离心机,离心柱
蛋白质形式重组
数量25柱
固定相NHS 酯
足够用于≥25 mg/mL 干树脂
靶标抗体、蛋白、肽、配体
容量(公制)0.2 mL
形式液体
产品线Pierce™
类型离心柱
Unit SizeEach
内容与储存
接收后,在- 20°C 下储存干树脂。产品装于冰袋中运输。

常见问题解答 (FAQ)

Can you provide the shelf-life for NHS-Activated Agarose Spin Columns?

NHS-Activated Agarose Spin Columns are covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does the NHS-activated agarose resin work, and what are its benefits?

NHS- activated agarose reacts with primary and secondary amines to form stable amide and imide linkages respectively. Most proteins can be coupled in 30 mins with greater than 85% coupling efficiency. No hazardous chemicals are used, and the agarose is adaptable to use with spin columns, batch methods, or FPLC. The resin is resusable typically up to 10 purifications with the immobilized ligand, and has a high binding capacity (>30 mg/mL slurry or >25 mg/mL dry resin).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.