Pierce™ NHS-Activated Agarose Spin Columns, 2 mL - FAQs

查看更多产品信息 Pierce™ NHS-Activated Agarose Spin Columns, 2 mL - FAQs (26199)

3 个常见问题解答

Can you provide the shelf-life for NHS-Activated Agarose Spin Columns?

NHS-Activated Agarose Spin Columns are covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does the NHS-activated agarose resin work, and what are its benefits?

NHS- activated agarose reacts with primary and secondary amines to form stable amide and imide linkages respectively. Most proteins can be coupled in 30 mins with greater than 85% coupling efficiency. No hazardous chemicals are used, and the agarose is adaptable to use with spin columns, batch methods, or FPLC. The resin is resusable typically up to 10 purifications with the immobilized ligand, and has a high binding capacity (>30 mg/mL slurry or >25 mg/mL dry resin).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.