384 Well White Plate, Non-Treated Surface, Non-Sterile, Pack of 25 - FAQs

Q: Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on top reader instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom. Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center .

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4 个常见问题解答

Can I use Nunc White 384-Well Microplates for fluorescent assays?

Nunc White 384-Well Microplates can be used for fluorescent applications, but background fluorescence will be much higher compared to Nunc Black 384-Well Microplates.
We recommend the white microplates for scintillation and luminescent detection systems, in which the reflective properties of the microplate enhance the signal without producing any endogenous background signal.

Do you offer Nunc Microplate Lids for the Nunc Black or White 384-Well Microplates?

We offer Nunc Microplate Lids: Cat. No. 448143, that fit both the 384-well microplates and the 1,536-well microplates.

Why do you offer Nunc 384-Well Black or White Microplates when the cells cannot be observed using an inverted light microscope?

We offer Nunc Black or White 384-Well microplates that are intended for use in conjunction with 384-well microplate luminometers and fluorometers that have excitation and detection systems located above the plate. Due to the opaque quality of these microplates, all detection systems must rely on either luminescent or fluorescent reagents, such as viability stains that report cell activity and membrane integrity.

Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on “top reader” instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.