TheraPure 2ʹ-Fluoro-iBu-G Phosphoramidite

2'-Fluoro-iBu-G CEP is a phosphoramidite containing 5' DMT and the nucleoside guanosine (G) with 2'-Fluoro protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, the highly electronegative fluorine (2'-Fluoro) modification adopts a more thermodynamically stable A-form helix on hybridization to its target. Showing improved affinity and some nuclease resistance (compared to 2'-OH), the 2'-F modification has been proven useful in antisense, siRNA, and aptamer applications. The 'standard grade' marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in an amber bottle.

Available with a Serum Vial, 20-400 Finish, or 28-400 Finish neck style.

• Serum vial neck style is compatible with most Applied Biosystems synthesizers and AKTA synthesizers.
• 20-400 finish neck style is compatible with most Expedite synthesizers.
• 28-400 finish neck style is compatible with most MerMade synthesizers.

Features:

• Full chemical name: 5'-O-(4,4'-Dimethoxytrityl)-N²-isobutyryl-2'-fluoroguanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula: C₄₄H₅₃FN₇O₈P
• Base: G
• Base Protection: iBu
• Formula weight: 857.4 g/mol
• CAS number: 144089-97-4
• HPLC purity: >98%
• ³¹P NMR purity: ≥98%