Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method.
AuthorsAraki Y, Hamafuji T, Noguchi C, Shimizu N,
JournalPLoS One
PubMed ID22844523
We previously found that plasmids bearing a mammalian replication initiation region (IR) and a nuclear matrix attachment region (MAR) efficiently initiate gene amplification and spontaneously increase their copy numbers in animal cells. In this study, this novel method was applied to the establishment of cells with high recombinant antibody production. ... More
Polyurethane surfaces modified by amphiphilic polymers: effects on protein adsorption.
AuthorsFreij-Larsson C, Jannasch P, Wesslén B,
JournalBiomaterials
PubMed ID10646948
Surface modification of polyurethane (PUR) surfaces was carried out by using three different amphiphilic polymers. Two of the polymers were graft copolymers, having backbones consisting of poly(methyl methacrylate-co-ethylhexyl acrylate) and poly(styrene-co-acrylamide), respectively, and poly(ethylene oxide) PEO 2000 grafts. The third polymer was a commercially available poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) block ... More