胰蛋白酶 (1:250)，粉末

通常情况下，胰酶可在冰箱中保存一周。不过，这一时间长度也随着这一周内的加热次数和使用频率而变化。同时，它也与细胞粘附的紧密程度有关，胰酶在非冰冻条件下每天都会丧失一定的活性。

TrypLE试剂不含动物源性与人源成份，纯度极高且对细胞的作用十分温和。该产品无需使用胰酶抑制剂对其进行终止。在含血清和不含血清的培养条件下，TrypLE试剂都是解离各类贴壁培养的哺乳动物细胞系的理想之选，能够直接做为胰酶替代品而应用于您当前的实验方案。

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

Trypsin (1:250), powder (Cat. No. 27250018) is prepared by adding the 0.25 g of Trypsin powder to 100 mL of chilled HBSS (Cat. No. 14170112) to create a 0.25% solution.

For 1% solution, you would add 1g of trypsin powder to 100 mL of chilled HBSS.

The solution should be mixed for approximately 10 minutes, and the pH should be between 7.4-7.8. The solution should be sterile filtered (0.22 µm filter) and stored -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend storing reconstituted Trypsin (1:250), powder (Cat. No. 27250018) at -20C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.