Nunc™ 96 孔过滤板

29.936 in. HG equals 1.013 bars
So -5 to -10 in. Hg equals -170 to -340 mbar and -15 to -20 in. Hg equals -500 to -680 mbar

Any vacuum manifold (for 96-well microplates) with an inside depth of 4 cm or more should be fine.

Yes. The polypropylene plate material naturally resists chemicals and organic solvents, as does the polyethylene terephthalate (PET) frit and glass fiber filter. For very strong chemicals, prolonged exposure times, and/or higher temperatures, we recommend that you test the plates with the chemical or solution in question.

The protocol is designed to be flexible, depending on customer needs and instrumentation. For example, certain steps can be performed with vacuum filtration, centrifugation, or positive pressure, with similar results. We do recommend that customers use our formulas for buffers and solutions, since they were optimized to produce superior results.