Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors.
AuthorsSong E, Zhu P, Lee SK, Chowdhury D, Kussman S, Dykxhoorn DM, Feng Y, Palliser D, Weiner DB, Shankar P, Marasco WA, Lieberman J,
JournalNat Biotechnol
PubMed ID15908939
'Delivery of small interfering RNAs (siRNAs) into cells is a key obstacle to their therapeutic application. We designed a protamine-antibody fusion protein to deliver siRNA to HIV-infected or envelope-transfected cells. The fusion protein (F105-P) was designed with the protamine coding sequence linked to the C terminus of the heavy chain ... More
Centaurin-{alpha}1 Is a Phosphatidylinositol 3-Kinase-dependent Activator of ERK1/2 Mitogen-activated Protein Kinases.
AuthorsHayashi H, Matsuzaki O, Muramatsu S, Tsuchiya Y, Harada T, Suzuki Y, Sugano S, Matsuda A, Nishida E,
JournalJ Biol Chem
PubMed ID16287813
Centaurin-alpha(1) is known to be a phosphatidylinositol 3,4,5-triphosphate (PIP(3))-binding protein that has two pleckstrin homology domains and a putative ADP ribosylation factor GTPase-activating protein domain. However, the physiological function of centaurin-alpha(1) is still not understood. Here we have shown that transient expression of centaurin-alpha(1) in COS-7 cells results in specific ... More