Raloxifene Upregulated Mesangial Cell MMP-2 Activity via ER-ß Through Transcriptional Regulation.
AuthorsFang M, Wu XC, Huang W,
JournalCell Biochem Biophys
PubMed ID23471663
'Raloxifene, a second-generation selective estrogen receptor modulator, exerts estrogen-like effects in specific tissues. In this present study, we examined the effect of raloxifene on mesangial cell matrix metalloproteinase-2 (MMP-2) activity in streptozotocin-induced diabetic mice. Raloxifene increased the MMP-2 level in a dose-dependent and receptor-mediated manner. An antibody against estrogen receptor-ß ... More
Single cell rt-PCR identification of odorant receptors expressed by olfactory neurons.
AuthorsMalnic B,
JournalMethods Mol Biol
PubMed ID23585038
'Mammals have between 400 and 1,300 functional odorant receptor (OR) genes in their genomes. Each olfactory sensory neuron in the nose expresses only one single type of OR out of this vast repertoire. The OR expressed by an olfactory sensory neuron determines its functional activity and wiring to the olfactory ... More
Fast-mode duplex qPCR for BCR-ABL1 molecular monitoring: innovation, automation, and harmonization.
AuthorsGerrard G, Mudge K, Foskett P, Stevens D, Alikian M, White HE, Cross NC, Apperley J, Foroni L,
JournalAm J Hematol
PubMed ID22566190
Reverse transcription quantitative polymerase chain reaction (RTqPCR)is currently the most sensitive tool available for the routine monitoring of disease level in patients undergoing treatment for BCRABL1 associated malignancies. Considerable effort has been invested at both the local and international levels to standardise the methodology and reporting criteria used to assess ... More
Transforming growth factor-ß is required for vasculogenic mimicry formation in glioma cell line U251MG.
AuthorsLing G, Wang S, Song Z, Sun X, Liu Y, Jiang X, Cai Y, Du M, Ke Y,
JournalCancer Biol Ther
PubMed ID22104964
Both vasculogenic mimicry (VM) and transforming growth factor-ß (TGFß) are positively correlated with malignancy in glioma. Accordingly, we supposed that TGFß might be related with VM, and aimed to detect whether TGFß could influence VM formation in two glioma cell lines U251MG and SHG44, which were different in malignancy. We ... More