胎牛血清，胚胎干细胞合格，新西兰来源

我们的研究结果显示在4°C条件下短时保存化冻的FBS，28天（之内）不会降低其生长或活性方面的效能。如需在4°C条件下将FBS保存更长时间，就需要逐案评估这一储存条件是否为必须。

多种原因可能导致FBS中出现絮状沉淀。最为常见的原因是血清中脂蛋白的变性。你可能会在血清化冻后观察到血纤蛋白——一种血清中存在的促凝蛋白。这并不会影响本品的使用性能。

Gibco FBS未经预老化处理。当储存于2至8°C的条件下，血清中可能存在的各类蛋白与脂蛋白（如冷凝集素，纤维蛋白原，玻连蛋白等）会发生聚集，并且形成肉眼可见的沉淀或混浊。这并不会影响血清的使用性能。我们推荐您将FBS储存于–20°C，同时避免反复冻融。

1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.