DMEM/F-12, HEPES
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DMEM/F-12, HEPES
Gibco™

DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth ofRead more
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Catalog NumberQuantity
31330095
also known as 31330-095
10 x 500 mL
11330032500 mL
31330038
also known as 31330-038
500 mL
1133005710 x 500 mL
113300995 L
1133010710 L
Catalog number 31330095
also known as 31330-095
Price (CNY)
5,743.00
Each
Add to cart
Quantity:
10 x 500 mL
Customize this product
Price (CNY)
5,743.00
Each
Add to cart

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With: L-glutamine, HEPES, Phenol Red

The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity10 x 500 mL
Shelf Life12 Months
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, HEPES, Phenol Red, High Glucose, Sodium Pyruvate
Unit SizeEach

Frequently asked questions (FAQs)

The lids of DMEM/F-12, HEPES medium (Cat. No. 31330038, 11330032, 31330095, 11330057, 11330107) have a different color now. Have there been any changes in the composition of the medium?

The color change of the lid has no effect on the sterility or functionality of the product. There has been no change to the end product DMEM/F-12, HEPES medium (Cat. No. 31330038, 11330032, 31330095, 11330057, 11330107); the medium still has the same composition.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.