Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro.
AuthorsMcGuckin C, Jurga M, Ali H, Strbad M, Forraz N,
JournalNat Protoc
PubMed ID18536651
'This 3-week protocol produces embryonic-like stem cells from human umbilical cord blood (CBEs) for neural differentiation using a three-step system (cell isolation/expansion/differentiation). The CBE isolation produces a highly purified fraction (CD45-, CD33-, CD7-, CD235a-) of small pluripotent stem cells (2-3 microm in diameter) coexpressing embryonic stem cell markers including Oct4 ... More