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Invitrogen
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP
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Mouse IgG (H+L) Secondary Antibody (31430) in WB
Chemiluminiscent western blot was performed using Goat anti-Mouse IgG (Heavy Chain) Secondary Antibody, HRP (Product # 31430). Whole cell extracts of U-87 MG (Lane 1, 2, 3, 4), SH-SY5Y (Lane 5, 6, 7, 8), HeLa (Lane 9) and Hep G2 (Lane 10) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03221BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB23001) b... View More
产品信息
31430
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:200,000
免疫组化（石蜡） (IHC (P))
1:500
酶联免疫吸附实验 (ELISA)
1:10,000-1:25,000
免疫沉淀 (IP)
1:500-1:5,000
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Lyophilized
浓度
0.8 mg/mL
规格
1.6 mg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Product # 31430 has been validated for use in Western blot, immunohistochemistry (IHC), ELISA, and immunoprecipitation (IP) applications.
This product reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Storage Conditions
Store at 2-8°C prior to reconstitution.
Reconstitution and Storage Guidelines
Short-term storage (≤1 week): Reconstitute with 2 mL distilled water. Store at 2-8°C.
Long-term storage: Reconstitute with 1 mL distilled water, add 1 mL glycerol, and store at -20°C. Stable for up to 1 year.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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