IMDM,GlutaMAX™ 添加剂
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Gibco™

IMDM,GlutaMAX™ 添加剂

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货号数量
31980048
又称 31980-048
10 x 500 mL
货号 31980048
又称 31980-048
价格(CNY)
-
数量:
10 x 500 mL
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Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of Gibco™ IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This IMDM is modified as follows:

With: GlutaMAX™, Phenol Red

Without: α-thioglycerol, 2-mercaptoethanol

The complete formulation is available.

IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. Gibco™ IMDM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

cGMP Manufacturing and Quality System

Gibco™ IMDM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ IMDM product made in our Grand Island facility (31980-097). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

规格
细胞类型哺乳动物细胞
最大浓度1 X
培养环境CO2
渗透压270 - 310 mOsm/kg
产品线Gibco™、GlutaMAX™
产品类型Iscove 改良型 Dulbecco 培养基 (IMDM)
数量10 x 500 mL
有效期12 个月
分类非动物源性
培养类型哺乳动物细胞培养
形式液体
无菌无菌过滤
加有添加剂GlutaMAX, HEPES:, 酚红, 丙酮酸钠, 碳酸氢钠
不加添加剂无 α±-硫代甘油, 无 2-巯基乙醇
pH6.9 - 7.3
Unit SizeEach
内容与储存
储存条件:2-8°C。避光储存。
运输条件:室温。
有效期:自生产之日起 12 个月。

常见问题解答 (FAQ)

清除,缺少或降解谷氨酰胺或生长因子等促生长的基本成份会对培养物的生长速率造成影响么?

是的。如果您怀疑发生了类似情况,请移去培养基并换用新的培养基。或者,您可以添加含有促生长成份的培养基。也可使用I或II型GlutaMAX取代培养基中的谷氨酰胺,以避免谷氨酰胺被耗竭。

 

配方中含有Gibco GlutaMAX添加剂会改变L-谷氨酰胺的含量么?

在所有以GlutaMAX双肽添加剂作为L-谷氨酰胺替代品的培养基中,谷氨酰胺的浓度都与原始配方中的L-谷氨酰胺浓度处于同等的摩尔水平。

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.