Opti-MEM™ I Reduced Serum Medium
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Opti-MEM™ I Reduced Serum Medium
Citations & References (5)
Gibco™

Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumRead more
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Catalog NumberQuantity
3198508810 x 500 mL
31985070500 mL
31985062100 mL
Catalog number 31985088
Price (CNY)
7,312.00
Each
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Quantity:
10 x 500 mL
Customize this product
Price (CNY)
7,312.00
Each
Add to cart

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.


This Opti-MEM™ I is modified as follows:
With
• L-glutamine
• Phenol Red


The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineSp2, AE-1, CHO, BHK-21, and HEK
Cell TypePrimary Fibroblasts
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco, Opti-MEM
Product TypeOpti-MEM I
Quantity10 x 500 mL
Shelf Life18 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationHuman Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red
Unit SizeEach
Contents & Storage
Store at 2–8°C and protect from light.

Frequently asked questions (FAQs)

What should I use to dilute my RNAi duplexes and Lipofectamine RNAiMAX before complexing?

We recommend using Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Do you offer an alternative for Opti-MEM I Reduced Serum Medium (Cat. No. 31985070)?

Opti-MEM I Reduced Serum Medium, no phenol red (Cat. No. 11058021) would be the closest alternative. Both offerings contain L-glutamine. For more a stabilized L-glutamine option, Opti-MEM I Reduced Serum Medium, GlutaMAX Supplement (Cat. No. 51985034) is the recommended alternative. All Opti-MEM offerings have demonstrated performance for both reduced-serum cell culture and lipid-based transfection.

My OpitMEM Reduced Serum Medium (Cat. No. 31985070) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ I Reduced Serum Medium, 10 x 500 mL FAQs

Citations & References (5)

Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Authors:Whitford Kristin L; Marillat Valérie; Stein Elke; Goodman Corey S; Tessier-Lavigne Marc; Chédotal Alain; Ghosh Anirvan;
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Cloning and characterization of freac-9 (FKHL17), a novel kidney-expressed human forkhead gene that maps to chromosome 1p32-p34.
Authors: Ernstsson S; Betz R; Lagercrantz S; Larsson C; Ericksson S; Cederberg A; Carlsson P; Enerbäck S;
Journal:Genomics
PubMed ID:9403061
'We describe the cloning of a near full-length cDNA of 4258 nucleotides encoding freac-9 (HGMW-approved symbol FKHL17), a novel human forkhead gene. The 5'' untranslated region is unusual since it is very long, 2127 nucleotides, and contains 15 upstream AUG codons. Hybridization to a panel consisting of RNA derived from ... More
Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
Authors:Sharma S, Murai F, Miyanohara A, Friedmann T
Journal:Proc Natl Acad Sci U S A
PubMed ID:9380714
Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse ... More
Human T-cell leukemia virus type I Tax protein transactivates RNA polymerase III promoter in vitro and in vivo.
Authors: Piras G; Dittmer J; Radonovich M F; Brady J N;
Journal:J Biol Chem
PubMed ID:8702791
Tax protein of the human T-cell lymphotropic virus type 1 (HTLV-I) is critical for viral replication and is a potent transcriptional activator of viral and cellular polymerase II (pol II) genes. We report here that Tax is able to transactivate a classical pol III promoter, VA-I. In cotransfection experiments, Tax ... More