Miser™ Antibody Extender Solution NC - FAQs

如果你对你的western信号满意，你可以使用Miser Extender Solution NC来减少你需要使用的抗体量，从而降低你的成本。如果你需要增加灵敏度，那么你可以使用Western Blot信号增强剂。

膜上的蛋白质更容易被抗体接触，使得使用较少抗体产生信号成为可能。

Miser Antibody Extender Solution应在使用丽春红染色后使用。

Miser Antibody Extender Solution在硝酸纤维素膜上能提供一致的、可重复的结果；在PVDF膜上的结果因膜而异。

Miser Antibody Extender Solution应在将蛋白质转膜后，封闭前使用。

If you are happy with your western signal, you can use the Miser Antibody Extender Solution NC to minimize the amount of antibody you need to use and therefore reduce your costs. If you need to increase sensitivity, then you can use the Pierce Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The proteins on the blot become more accessible to the antibodies, making it possible for a signal to be produced using less antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Extender Solution should be used after staining with Ponceau S.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Extender solution gives consistent, reproducible results on nitrocellulose membranes; results can vary with PVDF membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Extender Solution should be used after transferring the protein to the membrane and before blocking the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.