Pierce™ ECL Plus Western Blotting Substrate
Pierce™ ECL Plus Western Blotting Substrate
Thermo Scientific™

Pierce™ ECL Plus Western Blotting Substrate

Thermo Scientific的Pierce ECL Plus底物是一种用于印迹检测,基于9,10-二氢吖啶配方的化学发光及化学荧光HRP底物,可通过X射线胶片、CCD或激光成像仪进行成像。ECL Plus底物的特点:• 简单易用— 可替代停产的GE ECL了解更多信息
Have Questions?
更改视图buttonViewtableView
货号DimensionsLxWxH
32132
32134
32132X3
货号 32132
价格(CNY)
3,890.00
Each
添加至购物车
请求批量或定制报价
价格(CNY)
3,890.00
Each
添加至购物车
Thermo Scientific的Pierce ECL Plus底物是一种用于印迹检测,基于9,10-二氢吖啶配方的化学发光及化学荧光HRP底物,可通过X射线胶片、CCD或激光成像仪进行成像。

ECL Plus底物的特点:

简单易用— 可替代停产的GE ECL Plus底物,无需进行任何优化
灵敏度更高— 可检测低皮克级的蛋白
信号持续时间更长— 光信号持续时间长达5小时
更多成像方法— 适用于X射线胶片、CCD或激光成像仪
价格更经济— 相比其他品牌的类似产品,不仅具有高品质和高性能,同时价格也更低

Pierce ECL Plus底物与HRP反应会生成吖啶酯,酯类中间体会与过氧化氢发生反应,产生强烈并持久的化学发光信号,这些信号可通过X射线胶片或CCD成像仪捕获。Pierce ECL Plus底物还可以产生强烈的荧光信号,通过荧光成像仪进行捕获。使用适当的一抗及二抗检测时,Pierce ECL Plus底物可检测硝化纤维素膜或PVDF膜上低皮克级的目标蛋白。Pierce ECL Plus底物具备动态范围宽、灵敏度高以及信号持续时间长等特点,是印迹分析应用的最佳选择。

相关资源:
免疫印迹与检测技术指南
免疫印迹视频教程

相关产品:
化学发光免疫印迹底物选择指南
印迹设备、膜与信号增强剂
CL-XPosure胶片(用于化学发光的X射线胶片)
For Research Use Only. Not for use in diagnostic procedures.
规格
偶联物HRP
描述Pierce ECL Plus Western Blotting Substrate
套装内容Detection Reagent A, 100 mL, Detection Reagent B, 2.5 mL
灵敏度Low picogram to mid-femtogram
足够用于10 mini-gel blots, 1000 cm2 of membrane
检测方法Chemiluminescence, Fluorescence
适用于(应用)Protein Assay and Analysis
形式Liquid
膜兼容性Nitrocellulose, PVDF
产品线Pierce™
产品类型底物
数量100 mL Kit
Unit SizeEach
内容与储存
Upon receipt store at 4°C. Product shipped on ice.

常见问题解答 (FAQ)

SuperSignal West Dura化学发光底物比其他增强型化学发光(ECL)底物灵敏度高多少?

SuperSignal West Dura化学发光底物的灵敏度比GE Healthcare ECL底物或Thermo Scientific ECL底物(货号32106)高10倍。SuperSignal West Dura化学发光底物的灵敏度也显著高于碱性磷酸酶的化学发光底物。

How much more sensitive is SuperSignal West Dura Extended Duration Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.