For Research Use Only. Not for use in diagnostic procedures.
规格
偶联物HRP
描述Pierce ECL Plus Western Blotting Substrate
套装内容Detection Reagent A, 100 mL, Detection Reagent B, 2.5 mL
灵敏度Low picogram to mid-femtogram
足够用于10 mini-gel blots, 1000 cm2 of membrane
检测方法Chemiluminescence, Fluorescence
适用于(应用)Protein Assay and Analysis
形式Liquid
膜兼容性Nitrocellulose, PVDF
产品线Pierce™
产品类型底物
数量100 mL Kit
Unit SizeEach
内容与储存
Upon receipt store at 4°C. Product shipped on ice.
常见问题解答 (FAQ)
SuperSignal West Dura化学发光底物比其他增强型化学发光(ECL)底物灵敏度高多少?
SuperSignal West Dura化学发光底物的灵敏度比GE Healthcare ECL底物或Thermo Scientific ECL底物(货号32106)高10倍。SuperSignal West Dura化学发光底物的灵敏度也显著高于碱性磷酸酶的化学发光底物。
How much more sensitive is SuperSignal West Dura Extended Duration Substrate than the other enhanced chemiluminescent (ECL) substrates?
SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).
How can I increase the sensitivity of my Western blot?
Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.