Caspase-7 gene disruption reveals an involvement of the enzyme during the early stages of apoptosis.
AuthorsKorfali N, Ruchaud S, Loegering D, Bernard D, Dingwall C, Kaufmann SH, Earnshaw WC,
JournalJ Biol Chem
PubMed ID14583630
Caspases play a key role during apoptotic execution. In an attempt to elucidate the specific role of caspase-7 we generated a chicken DT40 cell line in which both alleles of the gene were disrupted. Viability assays showed that caspase-7-/- clones are more resistant to the common apoptosis-inducing drugs etoposide and ... More
Gap junction-microtubule associations in rat alveolar epithelial cells.
AuthorsGuo Y, Martinez-Williams C, Rannels DE,
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID14604851
Connexin 43 (Cx43) is a predominant gap junction (GJ) protein expressed by alveolar epithelial cells (AEC) in primary cell culture. Cx43 trafficking, assembly, and turnover are regulated by multiple mechanisms, including those mediated by integrins, by extracellular matrix, and by the cytoskeleton. Immunocytochemical double labeling demonstrates association of microtubules with ... More
Effects of proinflammatory cytokines on the claudin-19 rich tight junctions of human retinal pigment epithelium.
AuthorsPeng S, Gan G, Rao VS, Adelman RA, Rizzolo LJ,
JournalInvest Ophthalmol Vis Sci
PubMed ID22761260
Chronic, subclinical inflammation contributes to the pathogenesis of several ocular diseases, including age-related macular degeneration. Proinflammatory cytokines affect tight junctions in epithelia that lack claudin-19, but in the retinal pigment epithelium claudin-19 predominates. We examined the effects of cytokines on the tight junctions of human fetal RPE (hfRPE). hfRPE was ... More
A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase.
AuthorsAkileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM
JournalJ Biol Chem
PubMed ID11279182
The cell nucleus is structurally and functionally organized by the nuclear matrix. We have examined whether the nuclear cAMP-dependent protein kinase-anchoring protein AKAP95 contains specific signals for targeting to the subnuclear compartment and for interaction with other proteins. AKAP95 was expressed in mammalian cells and found to localize exclusively to ... More