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Invitrogen
alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor™ 488
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图: 1 / 21
alpha Tubulin Antibody (322588) in ICC/IF
Immunofluorescence analysis of Alpha tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha tubulin (B-5-1-2) Alexa Fluor 488 Mouse Monoclonal Antibody, (Product # 322588) at 2µg/mL in 0.1% BSA a... View More
产品信息
322588
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Sea urchin
已发表种属
Chimpanzee,
Fungi,
Human,
Mouse,
Xenopus
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
B-5-1-2
抗原
Sarkosyl-resistant filament from sea urchin sperm axonemes
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 4mg/mL BSA
内含物
0.1% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody reacts with all forms of alpha tubulin. The expected molecular weight is ~50.5 kDa. Reactivity is confirmed with NIH3T3 cells, rat brain, mouse testis, human Caco-2, HeLa3 and PtK23 cells.
Excitation/Emission (nm) for this Alexa Fluor 488™-conjugated antibody: 495/519
靶标信息
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; tubulin alpha-1; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 4; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2; tubulin, beta 2B; tubulin, beta polypeptide paralog; unnamed protein product
基因别名: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; OZEMA23; OZEMA24; PMGYSA; RGD1309427; RGD1565476; SPAX11; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B
UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) P68369, (Rat) P05210, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8
Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081
nucleotide binding
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
hydrolase activity
identical protein binding
macromolecular complex binding
metal ion binding
protein heterodimerization activity
double-stranded RNA binding
GTPase activity
ubiquitin protein ligase binding
enzyme binding
protein kinase binding
protein domain specific binding
molecular_function
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
neuron migration
startle response
intracellular protein transport
microtubule-based process
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
cerebral cortex development
neurogenesis
neuron differentiation
sperm motility
adult behavior
cytoskeleton-dependent intracellular transport
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
cell division
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
cellular response to interleukin-4
cytoskeleton organization
nervous system development
modulation of synaptic transmission
positive regulation of axon guidance
embryonic brain development
protein polymerization
metabolic process
biological_process
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