p38? and p38d kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.
AuthorsRisco A, del Fresno C, Mambol A, Alsina-Beauchamp D, MacKenzie KF, Yang HT, Barber DF, Morcelle C, Arthur JS, Ley SC, Ardavin C, Cuenda A,
JournalProc Natl Acad Sci U S A
PubMed ID22733747
On the basis mainly of pharmacological experiments, the p38a MAP kinase isoform has been established as an important regulator of immune and inflammatory responses. However, the role of the related p38? and p38d kinases has remained unclear. Here, we show that deletion of p38? and p38d impaired the innate immune ... More
A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway.
AuthorsLi Q, Ching AK, Chan BC, Chow SK, Lim PL, Ho TC, Ip WK, Wong CK, Lam CW, Lee KK, Chan JY, Chui YL,
JournalJ Biol Chem
PubMed ID15465831
'BRE, brain and reproductive organ-expressed protein, was found previously to bind the intracellular juxtamembrane domain of a ubiquitous death receptor, tumor necrosis factor receptor 1 (TNF-R1), and to down-regulate TNF-alpha-induced activation of NF-kappaB. Here we show that BRE also binds to another death receptor, Fas, and upon overexpression conferred resistance ... More
A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
AuthorsBelham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J
JournalJ Biol Chem
PubMed ID12840024
'The Nek family of protein kinases in humans is composed of 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. Nek6 (314AA) and Nek7 (303AA), 76% identical, have little ... More
Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes.
AuthorsHeilemann M, van de Linde S, Schüttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M,
JournalAngew Chem Int Ed Engl
PubMed ID18646237
Herein we show that conventional fluorophores (Alexa Fluor 647, Cy5) can be used as efficient photoswitchable fluorescent probes. These probes can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths without the need for an activator fluorophore. Conceptually analogous to stochastic ... More
Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells.
AuthorsCoso S, Zeng Y, Sooraj D, Williams ED,
JournalExp Cell Res
PubMed ID21839079
Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial ... More
Structural and functional similarities of calcium homeostasis modulator 1 (CALHM1) ion channel with connexins, pannexins, and innexins.
AuthorsSiebert AP, Ma Z, Grevet JD, Demuro A, Parker I, Foskett JK,
JournalJ Biol Chem
PubMed ID23300080
CALHM1 (calcium homeostasis modulator 1) forms a plasma membrane ion channel that mediates neuronal excitability in response to changes in extracellular Ca(2+) concentration. Six human CALHM homologs exist with no homology to other proteins, although CALHM1 is conserved across >20 species. Here we demonstrate that CALHM1 shares functional and quaternary ... More
Impaired fertility and FSH synthesis in gonadotrope-specific Foxl2 knockout mice.
AuthorsTran S, Zhou X, Lafleur C, Calderon MJ, Ellsworth BS, Kimmins S, Boehm U, Treier M, Boerboom D, Bernard DJ,
JournalMol Endocrinol
PubMed ID23340250
Impairments in pituitary FSH synthesis or action cause infertility. However, causes of FSH dysregulation are poorly described, in part because of our incomplete understanding of mechanisms controlling FSH synthesis. Previously, we discovered a critical role for forkhead protein L2 (FOXL2) in activin-stimulated FSH ß-subunit (Fshb) transcription in immortalized cells in ... More
Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach.
AuthorsTsai BP, Wang X, Huang L, Waterman ML,
JournalMol Cell Proteomics
PubMed ID21285413
Identification of proteins in RNA-protein complexes is an important step toward understanding regulation of RNA-based processes. Because of the lack of appropriate methodologies, many studies have relied on the creation of in vitro assembled RNA-protein complexes using synthetic RNA and cell extracts. Such complexes may not represent authentic RNPs as ... More
Colchicine attenuates left ventricular hypertrophy but preserves cardiac function of aortic-constricted rats.
AuthorsScopacasa BS, Teixeira VP, Franchini KG,
JournalJ Appl Physiol (1985)
PubMed ID12482768
To investigate the effects of colchicine on left ventricular (LV) function and hypertrophy (LVH) of rats subjected to constriction of transverse aorta (TAoC), we evaluated SO (sham operated, vehicle; n = 25), SO-T (sham operated, colchicine 0.4 mg/kg body wt ip daily; n = 38), TAoC (vehicle; n = 37), ... More
A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase.
AuthorsAkileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM
JournalJ Biol Chem
PubMed ID11279182
The cell nucleus is structurally and functionally organized by the nuclear matrix. We have examined whether the nuclear cAMP-dependent protein kinase-anchoring protein AKAP95 contains specific signals for targeting to the subnuclear compartment and for interaction with other proteins. AKAP95 was expressed in mammalian cells and found to localize exclusively to ... More