B23 interacts with PES1 and is involved in nucleolar localization of PES1.
AuthorsZhang J, Yang Y, Wu J
JournalActa Biochim Biophys Sin (Shanghai)
PubMed ID20011973
'PES1, the human homolog of zebrafish pescadillo, is a nucleolar protein that is essential for cell proliferation. We report herein that a nucleolar marker protein B23 physically interacts with PES1 and is involved in the nucleolar localization of PES1. In vivo interaction between B23 and PES1 was verified by co-immunoprecipitation ... More
Sumoylation induced by the Arf tumor suppressor: a p53-independent function.
AuthorsTago K, Chiocca S, Sherr CJ,
JournalProc Natl Acad Sci U S A
PubMed ID15897463
'The mouse p19(Arf) protein has both p53-dependent and p53-independent tumor-suppressive activities. Arf triggers sumoylation of many cellular proteins, including Mdm2 and nucleophosmin (NPM/B23), with which p19(Arf) physically interacts in vivo, and this occurs equally well in cells expressing or lacking functional p53. In an Arf-null NIH 3T3 cell derivative (MT-Arf ... More
Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.
'Like phosphorylation, the addition of O-linked beta-N-acetylglucosamine (O-GlcNAcylation) is a ubiquitous, reversible process that modifies serine and threonine residues on nuclear and cytoplasmic proteins. Overexpression of the enzyme that adds O-GlcNAc to target proteins, O-GlcNAc transferase (OGT), perturbs cytokinesis and promotes polyploidy, but the molecular targets of OGT that are ... More
Free cholesterol-loaded macrophages are an abundant source of tumor necrosis factor-alpha and interleukin-6: model of NF-kappaB- and map kinase-dependent inflammation in advanced atherosclerosis.
AuthorsLi Y, Schwabe RF, DeVries-Seimon T, Yao PM, Gerbod-Giannone MC, Tall AR, Davis RJ, Flavell R, Brenner DA, Tabas I,
JournalJ Biol Chem
PubMed ID15826936
Two key features of atherosclerotic plaques that precipitate acute atherothrombotic vascular occlusion (
AuthorsPiazza F, Manni S, Tubi LQ, Montini B, Pavan L, Colpo A, Gnoato M, Cabrelle A, Adami F, Zambello R, Trentin L, Gurrieri C, Semenzato G
JournalBMC Cancer
PubMed ID20920357
Glycogen Synthase Kinase-3 (GSK-3) a and ß are two serine-threonine kinases controlling insulin, Wnt/ß-catenin, NF-?B signaling and other cancer-associated transduction pathways. Recent evidence suggests that GSK-3 could function as growth-promoting kinases, especially in malignant cells. In this study, we have investigated GSK-3a and GSK-3ß function in multiple myeloma (MM). ... More
Proteomic analysis reveals hyperactivation of the mammalian target of rapamycin pathway in neurofibromatosis 1-associated human and mouse brain tumors.
AuthorsDasgupta B, Yi Y, Chen DY, Weber JD, Gutmann DH,
JournalCancer Res
PubMed ID15805275
Individuals with the tumor predisposition syndrome, neurofibromatosis 1 (NF1), are prone to development of nervous system tumors, including neurofibromas and pilocytic astrocytomas. Based on the ability of the NF1 gene product (neurofibromin) to function as a GTPase activating protein for RAS, initial biologically based therapies for NF1-associated tumors focused on ... More
Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function.
AuthorsKorgaonkar C, Hagen J, Tompkins V, Frazier AA, Allamargot C, Quelle FW, Quelle DE,
JournalMol Cell Biol
PubMed ID15684379
The ARF tumor suppressor is a nucleolar protein that activates p53-dependent checkpoints by binding Mdm2, a p53 antagonist. Despite persuasive evidence that ARF can bind and inactivate Mdm2 in the nucleoplasm, the prevailing view is that ARF exerts its growth-inhibitory activities from within the nucleolus. We suggest ARF primarily functions ... More
Cyclin G1 has growth inhibitory activity linked to the ARF-Mdm2-p53 and pRb tumor suppressor pathways.
AuthorsZhao L, Samuels T, Winckler S, Korgaonkar C, Tompkins V, Horne MC, Quelle DE,
JournalMol Cancer Res
PubMed ID12556559
Cyclin G1 is a p53-responsive gene that is induced in alternative reading frame (ARF)-arrested cells, yet its role in growth control is unclear. We tested its effects on growth and involvement in the ARF-Mdm2-p53 tumor suppressor pathway. We show that cyclin G1 interacts with ARF, Mdm2, and p53 in vitro ... More
RNA helicase DDX5 is a p53-independent target of ARF that participates in ribosome biogenesis.
AuthorsSaporita AJ, Chang HC, Winkeler CL, Apicelli AJ, Kladney RD, Wang J, Townsend RR, Michel LS, Weber JD
JournalCancer Res
PubMed ID21937682
The p19ARF tumor suppressor limits ribosome biogenesis and responds to hyperproliferative signals to activate the p53 checkpoint response. Although its activation of p53 has been well characterized, the role of ARF in restraining nucleolar ribosome production is poorly understood. Here we report the use of a mass spectroscopic analysis to ... More
The role of RPGR in cilia formation and actin stability.
AuthorsGakovic M, Shu X, Kasioulis I, Carpanini S, Moraga I, Wright AF
JournalHum Mol Genet
PubMed ID21933838
Mutations in the retinitis pigmentosa GTPase regulator (RPGR) protein cause one of the most common and severe forms of inherited retinal dystrophy. In spite of numerous studies, the precise function of RPGR remains unclear, as is the mechanism by which RPGR mutations cause retinal degeneration. We have analysed the function ... More