Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis.
AuthorsAuclair JR, Boggio KJ, Petsko GA, Ringe D, Agar JN
JournalProc Natl Acad Sci U S A
PubMed ID21098299
Amyotrophic lateral sclerosis (ALS) is a disorder characterized by the death of both upper and lower motor neurons and by 3- to 5-yr median survival postdiagnosis. The only US Food and Drug Administration-approved drug for the treatment of ALS, Riluzole, has at best, moderate effect on patient survival and quality ... More
Mutational analysis of N381, a key trimer contact residue in Tsr, the Escherichia coli serine chemoreceptor.
AuthorsGosink KK, Zhao Y, Parkinson JS
JournalJ Bacteriol
PubMed ID21965562
Chemoreceptors such as Tsr, the serine receptor, function in trimer-of-dimer associations to mediate chemotactic behavior in Escherichia coli. The two subunits of each receptor homodimer occupy different positions in the trimer, one at its central axis and the other at the trimer periphery. Residue N381 of Tsr contributes to trimer ... More
Crosslinking snapshots of bacterial chemoreceptor squads.
AuthorsStuddert CA, Parkinson JS
JournalProc Natl Acad Sci U S A
PubMed ID14769919
The team signaling model for bacterial chemoreceptors proposes that receptor dimers of different detection specificities form mixed trimers of dimers. These receptor "squads" then recruit the cytoplasmic signaling proteins CheA and CheW to form ternary signaling teams, which typically cluster at the poles of the cell. We devised cysteine-directed in ... More