Association of the human SUMO-1 protease SENP2 with the nuclear pore.
AuthorsHang J, Dasso M,
JournalJ Biol Chem
PubMed ID11896061
'SUMO-1 is a small ubiquitin-like protein that can be covalently conjugated to other proteins. A family of proteases catalyzes deconjugation of SUMO-1-containing species. Members of this family also process newly synthesized SUMO-1 into its conjugatable form. To understand these enzymes better, we have examined the localization and behavior of the ... More
Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development.
AuthorsYamaguchi T, Sharma P, Athanasiou M, Kumar A, Yamada S, Kuehn MR,
JournalMol Cell Biol
PubMed ID15923632
'The covalent modification of proteins by the small ubiquitin-like protein SUMO has been implicated in the regulation of numerous biological processes, including nucleocytoplasmic transport, genomic stability, and gene transcription. Sumoylation occurs by a multienzyme process similar to ubiquitination and, in Saccharomyces cerevisiae, is reversed by desumoylating enzymes encoded by the ... More
A pathway linking oxidative stress and the Ran GTPase system in progeria.
AuthorsDatta S, Snow CJ, Paschal BM,
Journal
PubMed ID24523287
'Maintaining the Ran GTPase at a proper concentration in the nucleus is important for nucleocytoplasmic transport. Previously we found that nuclear levels of Ran are reduced in cells from patients with Hutchinson-Gilford progeria syndrome (HGPS), a disease caused by constitutive attachment of a mutant form of lamin A (termed progerin) ... More
The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function.
AuthorsChow KH, Elgort S, Dasso M, Powers MA, Ullman KS,
Journal
PubMed ID24196834
Nuclear pore complexes are composed of ~30 different proteins, each present at the pore in multiple copies. Together these proteins create specialized channels that convey cargo between the cytoplasm and the nuclear interior. With the building blocks of nuclear pores identified, one challenge is to decipher how these proteins are ... More
SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
AuthorsJoseph J, Tan SH, Karpova TS, McNally JG, Dasso M,
JournalJ Cell Biol
PubMed ID11854305
RanGAP1 was the first documented substrate for conjugation with the ubiquitin-like protein SUMO-1. However, the functional significance of this conjugation has not been fully clarified. We sought to examine RanGAP1 behavior during mitosis. We found that RanGAP1 associates with mitotic spindles and that it is particularly concentrated at foci near ... More
Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358.
Authors Saitoh Hisato; Pizzi Maryann Delli; Wang Jian;
JournalJ Biol Chem
PubMed ID11709548
Ubc9, a conjugation enzyme for the ubiquitin-related modifier SUMO, is present predominantly in the nucleus and at the nuclear pore complex. The functional significance of its subcellular compartmentalization, however, remains to be elucidated. Here, we define a Pro-Glu-Asp-Ser-Thr-rich element containing 129 amino acid residues, designated IR1+2, on the human nucleoporin ... More