'Normal endothelial and epithelial cells undergo apoptosis when cell adhesion and spreading are disrupted, implying a critical role of focal adhesions in cell survival. Cten is a focal adhesion molecule of the tensin family. In contrast to other tensins, cten expression is limited to very few tissues, such as the ... More
Enhanced ubiquitylation and accelerated degradation of the dopamine transporter mediated by protein kinase C.
AuthorsMiranda M, Wu CC, Sorkina T, Korstjens DR, Sorkin A,
JournalJ Biol Chem
PubMed ID16109712
'Dopamine transporter (DAT) localization in dopaminergic neurons plays an important role in regulating dopamine signaling. However, the mechanisms of DAT trafficking that control DAT localization are still poorly understood. To gain insight into these mechanisms, human DAT was purified in large amounts using a two-step affinity chromatography procedure from untreated ... More
giant nuclei is essential in the cell cycle transition from meiosis to mitosis.
At the transition from meiosis to cleavage mitoses, Drosophila requires the cell cycle regulators encoded by the genes, giant nuclei (gnu), plutonium (plu) and pan gu (png). Embryos lacking Gnu protein undergo DNA replication and centrosome proliferation without chromosome condensation or mitotic segregation. We have identified the gnu gene encoding ... More
Self-association of IQGAP1: characterization and functional sequelae.
AuthorsRen JG, Li Z, Crimmins DL, Sacks DB,
JournalJ Biol Chem
PubMed ID16105843
The scaffolding protein IQGAP1 participates in numerous cellular functions by binding to target proteins such as actin, calmodulin, E-cadherin, beta-catenin, Cdc42, Rac1, and CLIP-170. IQGAP1 regulates the cytoskeleton, promotes cell motility, and modulates E-cadherin-mediated cell-cell adhesion. However, how IQGAP1 exerts its functions in vivo is still unclear. In this study ... More