Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.
AuthorsShahidullah M, Mandal A, Beimgraben C, Delamere NA,
JournalJ Cell Physiol
PubMed ID21618533
'Purinergic receptors in lens epithelium suggest lens function can be altered by chemical signals from aqueous humor or the lens itself. Here we show release of ATP by intact porcine lenses exposed to hyposmotic solution (200 mOsm). 18a-glycyrrhetinic acid (AGA) added together with probenecid eliminated the ATP increase. N-ethylmaleimide (200 ... More
Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels.
AuthorsZampighi GA, Planells AM, Lin D, Takemoto D,
JournalInvest Ophthalmol Vis Sci
PubMed ID16123426
Lens fiber gap junctions comprise approximately equal molar amounts of connexin46 (Cx46) and connexin50 (Cx50), both of which contribute significantly to coupling in the lens cortex and nucleus. The current study was conducted to test the hypothesis that regulation of lens coupling by activation of protein kinase Cgamma (PKCgamma) affects ... More
Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.
AuthorsLin D, Lobell S, Jewell A, Takemoto DJ,
JournalMol Vis
PubMed ID15467523
Fiber cell gap junction proteins connexin 46 (Cx46) and connexin 50 (Cx50) play distinct roles in the avascular lens. The purpose of this study was to determine how protein kinase Cgamma (PKCgamma) differentially regulates phosphorylation of Cx46 and Cx50 in oxidatively stressed lenses. Sprague Dawley rats (six week old) were ... More
Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.
AuthorsKlepeis VE, Cornell-Bell A, Trinkaus-Randall V
JournalJ Cell Sci
PubMed ID11739651
This paper characterizes the early responses of epithelial cells to injury. Ca2+ is an important early messenger that transiently increases in the cytoplasm of cells in response to external stimuli. Its elevation leads to the regulation of signaling pathways responsible for the downstream events important for wound repair, such as ... More