DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line.
AuthorsWood RJ, Tchack L, Angelo G, Pratt RE, Sonna LA,
JournalPhysiol Genomics
PubMed ID14996990
The full extent to which 1,25-dihydroxyvitamin D(3) affects gene expression in human intestinal epithelial cells is unknown. We used oligonucleotide arrays to catalog vitamin D-induced changes in gene expression in Caco-2 cells, a human colon carcinoma cell line. Five paired sets of Caco-2 cell cultures were subjected to either control ... More
Identification and proteomic profiling of exosomes in human urine.
AuthorsPisitkun T, Shen RF, Knepper MA,
JournalProc Natl Acad Sci U S A
PubMed ID15326289
Urine provides an alternative to blood plasma as a potential source of disease biomarkers. One urinary biomarker already exploited in clinical studies is aquaporin-2. However, it remains a mystery how aquaporin-2 (an integral membrane protein) and other apical transporters are delivered to the urine. Here we address the hypothesis that ... More
Sorcin regulates excitation-contraction coupling in the heart.
AuthorsMeyers MB, Fischer A, Sun YJ, Lopes CM, Rohacs T, Nakamura TY, Zhou YY, Lee PC, Altschuld RA, McCune SA, Coetzee WA, Fishman GI,
JournalJ Biol Chem
PubMed ID12754254
Sorcin is a penta-EF hand Ca2+-binding protein that associates with both cardiac ryanodine receptors and L-type Ca2+ channels and has been implicated in the regulation of intracellular Ca2+ cycling. To better define the function of sorcin, we characterized transgenic mice in which sorcin was overexpressed in the heart. Transgenic mice ... More
Sorcin associates with the pore-forming subunit of voltage-dependent L-type Ca2+ channels.
AuthorsMeyers MB, Puri TS, Chien AJ, Gao T, Hsu PH, Hosey MM, Fishman GI
JournalJ Biol Chem
PubMed ID9668070
Intracellular Ca2+ release in muscle is governed by functional communication between the voltage-dependent L-type Ca2+ channel and the intracellular Ca2+ release channel by processes that are incompletely understood. We previously showed that sorcin binds to cardiac Ca2+ release channel/ryanodine receptors and decreases channel open probability in planar lipid bilayers. In ... More