The Toxoplasma gondii protein ROP2 mediates host organelle association with the parasitophorous vacuole membrane.
AuthorsSinai AP, Joiner KA
JournalJ Cell Biol
PubMed ID11448993
'Toxoplasma gondii replicates within a specialized vacuole surrounded by the parasitophorous vacuole membrane (PVM). The PVM forms intimate interactions with host mitochondria and endoplasmic reticulum (ER) in a process termed PVM-organelle association. In this study we identify a likely mediator of this process, the parasite protein ROP2. ROP2, which is ... More
Elevated AKT activity protects the prostate cancer cell line LNCaP from TRAIL-induced apoptosis.
AuthorsNesterov A, Lu X, Johnson M, Miller GJ, Ivashchenko Y, Kraft AS,
JournalJ Biol Chem
PubMed ID11278284
We find that the prostate cancer cell lines ALVA-31, PC-3, and DU 145 are highly sensitive to apoptosis induced by TRAIL (tumor-necrosis factor-related apoptosis-inducing ligand), while the cell lines TSU-Pr1 and JCA-1 are moderately sensitive, and the LNCaP cell line is resistant. LNCaP cells lack active lipid phosphatase PTEN, a ... More
Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.
O-linked GlcNAc transferase (OGT) mediates a novel glycan-dependent signaling pathway, but the intracellular targeting of OGT is poorly understood. We examined the localization of OGT by immunofluorescence microscopy, subcellular fractionation and immunoblotting using highly specific affinity-purified antisera. In addition to the expected nuclear localization, we found that OGT was highly ... More