Invitrogen

SRSF3 Monoclonal Antibody (1H4G7)

Name: SRSF3 Monoclonal Antibody (1H4G7)
Brand: Invitrogen
SKU: 33-9400
Price: 4960.00 CNY
Availability: InStock

高级验证

9个已发表图片

14 篇参考文献

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说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

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SRSF3 Antibody (33-9400) in WB

Western blot of HeLa cell lysates using Mouse anti-SR Proteins monoclonal Antibody (Product # 33-9400).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SRSF3 Antibody — Figure 6 Digoxin alters the activity of SR protein kinases and induces modification of a subset of SR proteins. (A) To assess the effect of digoxin on CLK kinase function, HeLa rtTA-HIV-Delta Mls cells were transfected with vectors expressing GFP-tagged CLK1. Twenty-four hours post-transfection, cells were treated with DMSO or 100 nM digoxin. After 24 h of treatment, cells were fixed, stained for SC35/SRSF2 nuclear speckles (Texas Red), and nuclei stained with DAPI. Images are representative of the localization patterns observed from >=5 independent experiments. Magnification 630x. (B, C, D) Western blot analysis of the effect of digoxin treatment on SR proteins. Lysates were harvested from HeLa rtTA-HIV-Delta Mls cells treated with 100 nM digoxin or DMSO and were induced with Dox (+) or left uninduced (-) as described in Fig. 1 . Immunoblots were probed by specific antibody for (B) phospho-SR proteins (1H4) or (C) SRp20, Tra2beta1, 9G8, or SF2/ASF (SF2). (D) Cell lysates from above were dephosphorylated by treatment with (+) and without (-) calf intestinal alkaline phosphatase (CIP), then immunoblotted with a SRp20-specific antibody. Arrows on the right indicate the protein specie(s) detected and their modified/unmodified isoform(s). Anti-tubulin (Anti-Tub.) blots for each experiment served as an internal loading control for the relative amount of protein lysate per lane. Immunoblots are representative of >=4 independent trials. {TM}

Immunogen image

Immunogen Image SRSF3 Monoclonal Antibody (1H4G7)

产品信息

33-9400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看9篇已发表文章 9篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Xenopus

已发表种属

Hamster, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1H4G7

抗原

Mass-isolated nuclei from Xenopus Laevis oocytes.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

Ambient (domestic); Wet ice (international)

AB_2533150

靶标信息

SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: epididymis secretory sperm binding protein; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; RNA binding protein; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD; unnamed protein product

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基因别名: SFRS3; SRp20

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UniProt ID: (Human) P84103

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Entrez Gene ID: (Human) 6428

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功能

nucleic acid binding RNA binding mRNA binding protein binding pre-mRNA binding phospholipase binding primary miRNA binding protein-RNA sequence-specific adaptor activity sequence-specific mRNA binding

参与通路

regulation of alternative mRNA splicing, via spliceosome RNA processing mRNA export from nucleus primary miRNA processing regulation of mRNA splicing, via spliceosome

SRSF3 Monoclonal Antibody (1H4G7)

SRSF3 Antibody (33-9400) in WB

产品信息

33-9400

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

规格

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

SRSF3 Monoclonal Antibody (1H4G7)

SRSF3 Antibody (33-9400) in WB

产品信息

33-9400

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

规格

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)