1-Step™ Ultra TMB-ELISA 底物溶液
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1-Step™ Ultra TMB-ELISA 底物溶液
Thermo Scientific™

1-Step™ Ultra TMB-ELISA 底物溶液

Thermo Scientific Pierce 1-Step Ultra TMB ELISA 底物 (1 L)了解更多信息
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货号数量底物
34028250 mLUltra TMB-ELISA Substrate
34024250 mLSlow TMB-ELISA Substrate
34022250 mLTurbo TMB-ELISA Substrate
340291 LUltra TMB-ELISA Substrate
34021400 mL 试剂盒TMB Substrate
货号 34028
价格(CNY)
2,228.00
飞享价
Ends: 31-Dec-2025
2,923.00
共减 695.00 (24%)
Each
添加至购物车
数量:
250 mL
底物:
Ultra TMB-ELISA Substrate
请求批量或定制报价
价格(CNY)
2,228.00
飞享价
Ends: 31-Dec-2025
2,923.00
共减 695.00 (24%)
Each
添加至购物车
Thermo Scientific Pierce 1-Step Ultra TMB ELISA 底物 (1 L) 可检测辣根过氧化物酶 (HRP) 活性,产生蓝色(Amax = 370 nm 和 652nm),加入硫酸或磷酸终止液后变为黄色 (Amax = 450 nm)。1-Step Ultra TMB 是极灵敏的发色底物。

1-Step Ultra TMB ELISA 底物的特点:

• 提供传统或即用型配方
• 无需额外的试剂或过滤
• 无致癌性
• 具有多种灵敏度水平和开发速度,可满足任何检测要求
• 试剂中不存在 DMF 或 DMSO

TMB 是 ELISA 中 HRP 检测的较常用显色底物,且可提供多种规格。Pierce 1-Step TMB 底物为单组分底物,使用前无需制备。与其他市售底物不同,这些产品不含 DMF 或 DMSO。
TMB 底物中 Thermo Scientific Pierce 1-Step Ultra TMB 的灵敏度较高,其次是 1-Step Turbo TMB 和 1-Step Slow TMB。1-Step Turbo TMB 的灵敏度与以约 1 mg/mL 配制的基于 OPD 的底物相似。1-Step Slow TMB 具有中等灵敏度— 灵敏度高于 ABTS,但低于邻苯二胺二盐酸盐 (OPD) 或 1-Step Turbo TMB。1-Step Slow TMB 是动力学研究的理想底物。

TMB(3,3',5,5'-四甲基联苯胺)是一种发色剂,在被氧化时产生蓝色,通常是由 HRP 水解过氧化氢产生的氧自由基所致。对于动态或未停止的 ELISA 试验,TMB 发色剂的上限吸收波长位于 370 nm 和 652 nm 处。然后在加入硫酸或磷酸时,颜色变为黄色,上限吸收波长位于 450 nm 处。绿色反应产物可能由蓝色中间产物部分转化为黄色产物而生成。TMB 是非常灵敏的 ELISA 底物,且与其他 HRP 底物相比会被更快氧化,因此可更快显色。

相关产品
Pierce™ TMB 底物试剂盒
1-Step™ Turbo TMB-ELISA 底物溶液
1-Step™ Slow TMB-ELISA 底物溶液
For Research Use Only. Not for use in diagnostic procedures.

1-Step 底物具有灵敏度不同的三种配方:

  • 1-Step Slow TMB 具有中等灵敏度 – 灵敏度高于 ABTS,但低于 OPD 或 1-Step Turbo TMB。
  • 1-Step Turbo TMB 的灵敏度与约 1 mg/mL OPD 的灵敏度相当
  • 1-Step Ultra TMB-ELISA 在皮克级范围内具有极高的信噪比和灵敏度

参考文献:

  1. Josephy, P.D., et al.(1982).J. Biol.Chem.257(7), 3669-3675.
  2. Josephy, P.D., et al.(1983).J. Biol.Chem.258(9), 5561-5569.
  3. Liem, H.H., et al.(1979).Anal.Biochem.98, 388-393.
规格
检测方法比色法
产品线1-Step™
运输条件室温
底物Ultra TMB-ELISA Substrate
底物属性化学底物
底物类型HRP(辣根过氧化物酶)
产品类型ELISA 底物
数量250 mL
Unit SizeEach
内容与储存
储存:接收后在 4°C 下防潮储存。产品在环境温度下运输。

常见问题解答 (FAQ)

What is the shelf-life of 1-Step Ultra TMB-ELISA Substrate Solution?

The 1-Step Ultra TMB-ELISA Substrate Solution is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My lab doesn't have a fluorescent or chemiluminescent plate reader and I need the most sensitive substrate for Horseradish Peroxidase (HRP). Which of your colorimetric substrates should I use?

The 1-Step Ultra TMB-ELISA is our most sensitive substrate for HRP in plate-based assays. It can detect down to ~ 2 pg/well or 20 pg/mL.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the 1-Step Ultra TMB-ELISA Substrate Solution be used as a precipitating chromogen for Western Blot, Immunohistochemistry and immunocytochemistry?

No. This product is formulated to generate a soluble, colored product that absorbs light at 450 nm. When used properly, there will be no precipitate, therefore, this substrate is only appropriate for plate-based assays, such as ELISA.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the 1-Step Ultra TMB-ELISA Substrate Solution an appropriate substrate for kinetic assays?

No. Although the non-stopped substrate will turn a blue color in the presence of Horseradish Peroxidase and absorb light at 307 nm and 652 nm, color development proceeds very quickly. In the time that it takes to read the full plate, the absorbance of the first wells will have changed. It is better to stop the the color development in all wells at approximately the same time point with an acidic soluton, turning it yellow and then read the absorbance at 450 nm.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.