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规格
检测方法比色法
产品线1-Step™
运输条件室温
底物Ultra TMB-ELISA Substrate
底物属性化学底物
底物类型HRP(辣根过氧化物酶)
产品类型ELISA 底物
数量250 mL
Unit SizeEach
内容与储存
储存:接收后在 4°C 下防潮储存。产品在环境温度下运输。
常见问题解答 (FAQ)
What is the shelf-life of 1-Step Ultra TMB-ELISA Substrate Solution?
The 1-Step Ultra TMB-ELISA Substrate Solution is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
My lab doesn't have a fluorescent or chemiluminescent plate reader and I need the most sensitive substrate for Horseradish Peroxidase (HRP). Which of your colorimetric substrates should I use?
The 1-Step Ultra TMB-ELISA is our most sensitive substrate for HRP in plate-based assays. It can detect down to ~ 2 pg/well or 20 pg/mL.
Can the 1-Step Ultra TMB-ELISA Substrate Solution be used as a precipitating chromogen for Western Blot, Immunohistochemistry and immunocytochemistry?
No. This product is formulated to generate a soluble, colored product that absorbs light at 450 nm. When used properly, there will be no precipitate, therefore, this substrate is only appropriate for plate-based assays, such as ELISA.
Is the 1-Step Ultra TMB-ELISA Substrate Solution an appropriate substrate for kinetic assays?
No. Although the non-stopped substrate will turn a blue color in the presence of Horseradish Peroxidase and absorb light at 307 nm and 652 nm, color development proceeds very quickly. In the time that it takes to read the full plate, the absorbance of the first wells will have changed. It is better to stop the the color development in all wells at approximately the same time point with an acidic soluton, turning it yellow and then read the absorbance at 450 nm.