SuperSignal™ West Pico Chemiluminescent Substrate - FAQs

不可以，这些SuperSignal底物需要不同的稀释方法。如果您将SuperSignal West Pico化学发光底物实验方案用于SuperSignal West Femto化学发光底物，将导致高背景或信号强度降低和/或持续时间缩短。SuperSignal West Femto化学发光底物必须使用较少的（较稀的）一抗和二抗（见产品说明书中的推荐范围）。

No, these SuperSignal substrates require different dilutions. If you follow the SuperSignal West Pico Chemiluminescent Substrate protocol when you use SuperSignal West Femto Maximum Sensitivity Substrate, you will see high background or a decreased signal intensity and or duration. You must use less (more dilute) primary and secondary antibodies with SuperSignal West Femto Maximum Sensitivity Substrate (see product instructions for recommended ranges).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

No, these SuperSignal substrates require different dilutions. If you follow the SuperSignal West Pico Chemiluminescent Substrate protocol when you use SuperSignal West Dura Extended Duration Substrate, you will see high background or a decreased signal intensity and or duration. You must use less (more dilute) primary and secondary antibodies with SuperSignal West Dura Extended Duration Substrate (see product instructions for recommended ranges).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

SuperSignal West Pico PLUS Chemiluminescent Substrate has a higher sensitivity and greater forgiveness for broad dilution range compared to SuperSignal West Pico Chemiluminescent Substrate. Please also see Tech Tip # 32 Guide to Enzyme Substrates for Western Blotting (https://tools.thermofisher.com/content/sfs/brochures/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.