SuperSignal™ West Femto Maximum Sensitivity Substrate - FAQs

Nitrocellulose and PVDF membranes can be stripped following detection with SuperSignal chemiluminescent substrates, for reprobing. Restore Western Blot Stripping Buffer (Cat. No. 21059, 21063) can be used to gently but efficiently remove probes from the western blot membrane without damaging the target protein. In most cases, re-blocking of the membrane is not necessary.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The following are possible causes for weak signal intensity:
- Low level of protein transferred from the gel to the membrane. This is particularly true for high molecular weight proteins. Reversible Protein Stain Kits for Nitrocellulose and PVDF membranes (Cat. Nos. 24580 and 24585, respectively) may be used to stain the bands on the membrane to indicate transfer efficiency.
- Over-diluted primary and secondary antibody probes. We suggest using the recommended dilution for the antibody probes for each substrate. More sensitive substrates will require a more dilute antibody solution.
- Short substrate incubation time. For best results, incubate the membrane for 5-15 min in the SuperSignal substrate.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.