MCM4 shares homology to a replication/DNA-binding domain in CTF and is contacted by pRb.
AuthorsSchmitz NM, Leibundgut K, Hirt A,
JournalBiochem Biophys Res Commun
PubMed ID15081408
pRb limits the initiation of DNA replication. By immunoblotting of in vitro kinase assays we show that Cdk4,6-cyclin D contributed to pRb-phosphorylation at Ser-608, which was necessary to release pRb from chromatin in Nalm-6 cells, demonstrated by immunoprecipitations of differential cell extractions. A new binding protein of under-phosphorylated pRb, MCM4, ... More