Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.
AuthorsChang TL, Ito K, Ko TK, Liu Q, Salto-Tellez M, Yeoh KG, Fukamachi H, Ito Y,
JournalGastroenterology
PubMed ID19706291
'The transcription factor RUNX3 is a gastric tumor suppressor. Tumorigenic Runx3(-/-) gastric epithelial cells attach weakly to each other, compared with nontumorigenic Runx3(+/+) cells. We aimed to identify RUNX3 target genes that promote cell-cell contact to improve our understanding of RUNX3''s role in suppressing gastric carcinogenesis. We compared gene expression ... More
Differential expression of claudin tight junction proteins in the human cortical nephron.
AuthorsKirk A, Campbell S, Bass P, Mason J, Collins J
JournalNephrol Dial Transplant
PubMed ID20124215
In renal tubules, paracellular permeability is tightly controlled to facilitate solute absorption and urinary concentration and is regulated by tight junctions, which incorporate claudin proteins. There is very limited information confirming the localization of these proteins in the human renal cortex. Most data is inferred from mouse, bovine and rabbit ... More
Tight Junctional Localization of Claudin-16 is Regulated by Syntaxin 8 in Renal Tubular Epithelial Cells.
AuthorsIkari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J,
Journal
PubMed ID24659781
Claudin-16 (CLDN16) regulates the paracellular reabsorption of Mg(2+) in the thick ascending limb (TAL) of Henle's loop. However, the mechanism regulating the tight junctional localization of CLDN16 remains unknown. In yeast two-hybrid systems, we found that CLDN16 bound to syntaxin 8 (STX8), a target soluble N-ethylmaleimide-sensitive factor attachment protein receptor. ... More
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
AuthorsCollins JE, Kirk A, Campbell SK, Mason J, Wilson SJ,
JournalMethods Mol Biol
PubMed ID21717371
There are a number of disadvantages with conventional tissue immunohistochemistry for accurate -localisation of claudin proteins. Traditionally, tissue cryopreservation or formaldehyde fixation with wax embedding is utilised prior to sectioning and antibody localisation. Wax embedding gives better morphological preservation than frozen tissue, but the required use of chemical cross-linking fixatives ... More