Functional characterization and localization of a gill-specific claudin isoform in Atlantic salmon.
AuthorsEngelund MB, Yu AS, Li J, Madsen SS, Færgeman NJ, Tipsmark CK
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID21975646
'Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is associated with remodeling of the epithelium during salinity change. This ... More
Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.
AuthorsChang TL, Ito K, Ko TK, Liu Q, Salto-Tellez M, Yeoh KG, Fukamachi H, Ito Y,
JournalGastroenterology
PubMed ID19706291
'The transcription factor RUNX3 is a gastric tumor suppressor. Tumorigenic Runx3(-/-) gastric epithelial cells attach weakly to each other, compared with nontumorigenic Runx3(+/+) cells. We aimed to identify RUNX3 target genes that promote cell-cell contact to improve our understanding of RUNX3''s role in suppressing gastric carcinogenesis. We compared gene expression ... More
Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.
'Tight junctions are essential for the maintenance of epithelial cell polarity. We have previously established a system for the primary culture of salivary parotid acinar cells that retain their ability to generate new secretory granules and to secrete proteins in a signal-dependent manner. Because cell polarity and cell-cell adhesion are ... More
Heterogeneity for stem cell-related markers according to tumor subtype and histologic stage in breast cancer.
AuthorsPark SY, Lee HE, Li H, Shipitsin M, Gelman R, Polyak K
JournalClin Cancer Res
PubMed ID20103682
To evaluate the expression of stem cell-related markers at the cellular level in human breast tumors of different subtypes and histologic stage.
Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells.
AuthorsYu AS, McCarthy KM, Francis SA, McCormack JM, Lai J, Rogers RA, Lynch RD, Schneeberger EE,
JournalAm J Physiol Cell Physiol
PubMed ID15689410
The function of occludin (Occ) in the tight junction is undefined. To gain insight into its role in epithelial cell biology, occludin levels in Madin-Darby canine kidney II cells were suppressed by stably expressing short interfering RNA. Suppression of occludin was associated with a decrease in claudins-1 and -7 and ... More
Inducible expression of Snail selectively increases paracellular ion permeability and differentially modulates tight junction proteins.
AuthorsCarrozzino F, Soulié P, Huber D, Mensi N, Orci L, Cano A, Féraille E, Montesano R,
JournalAm J Physiol Cell Physiol
PubMed ID15930145
Constitutive expression of the transcription factor Snail was previously shown to trigger complete epithelial-mesenchymal transition (EMT). The aim of this study was to determine whether inducible expression of Snail could modify epithelial properties without eliciting full mesenchymal conversion. For this purpose, we expressed mouse Snail (mSnail) cDNA in Madin-Darby canine ... More
Claudin 1 expression characterizes human uterine cervical reserve cells.
AuthorsZinner B, Gyöngyösi B, Babarczi E, Kiss A, Sobel G,
Journal
PubMed ID23900598
Stem cells participate in cervical carcinogenesis but their function and exact features are still not clear. One type of stem-like cells are endocervical reserve cells (RCs), and their association with other normal/altered cervical cells is not exactly known. Epithelial cells are attached to each other by tight junctions. Their dominant ... More
Claudin-1, -2, -3, -4, -7, -8, and -10 protein expression in biliary tract cancers.
AuthorsNémeth Z, Szász AM, Tátrai P, Németh J, Gyorffy H, Somorácz A, Szíjártó A, Kupcsulik P, Kiss A, Schaff Z,
JournalJ Histochem Cytochem
PubMed ID18854598
Biliary tract cancers are relatively common malignant gastrointestinal tumors in the elderly. Claudins are integral components of tight junctions that play important roles in maintaining epithelial cell polarity, controlling paracellular diffusion, and regulating cell growth and differentiation. The expression profile of claudins has been extensively characterized, but few reports exist ... More
Claudins 1, 2, 3, 4, 5 and 7 in solar keratosis and squamocellular carcinoma of the skin.
AuthorsHintsala HR, Siponen M, Haapasaari KM, Karihtala P, Soini Y,
Journal
PubMed ID24294371
Claudins are tight junction proteins regulating the paracellular permeability of cell layers. We investigated the expression of claudins 1, 2, 3, 4, 5 and 7 in a sample set consisting of a total of 93 cases representing normal skin, actinic keratoses and squamous cell carcinomas of the skin. There were ... More
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
AuthorsGuillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, Jond L, Bongiovanni M, Citi S,
JournalJ Cell Sci
PubMed ID22946046
Cingulin (CGN) is a 140 kDa protein, which is localized to the cytoplasmic region of vertebrate tight junctions (TJ), and regulates gene expression and RhoA signaling in cultured cells. To investigate the function of CGN at the organism level, we generated CGN knockout (CGN(-/-)) mice by homologous recombination. CGN(-/-) mice ... More
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
AuthorsCollins JE, Kirk A, Campbell SK, Mason J, Wilson SJ,
JournalMethods Mol Biol
PubMed ID21717371
There are a number of disadvantages with conventional tissue immunohistochemistry for accurate -localisation of claudin proteins. Traditionally, tissue cryopreservation or formaldehyde fixation with wax embedding is utilised prior to sectioning and antibody localisation. Wax embedding gives better morphological preservation than frozen tissue, but the required use of chemical cross-linking fixatives ... More