GlutaMAX™ Supplement, 100 mL - FAQs

是的。如果您怀疑发生了类似情况，请移去培养基并换用新的培养基。或者，您可以添加含有促生长成份的培养基。也可使用I或II型GlutaMAX取代培养基中的谷氨酰胺，以避免谷氨酰胺被耗竭。

在所有以GlutaMAX双肽添加剂作为L-谷氨酰胺替代品的培养基中，谷氨酰胺的浓度都与原始配方中的L-谷氨酰胺浓度处于同等的摩尔水平。

GlutaMAX-I 添加剂是一款能够改善细胞培养效果的添加剂，是您细胞培养基中L-谷氨酰胺的直接替代品。请点击此处(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html)来阅读更多关于GlutaMAX添加剂性能的详细信息。

GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.

Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The formulation and manufacturing process are the same for all three products. The main difference is in the “intended use” statement. CTS GlutaMAX-I Supplement (Cat. No. A1286001) and CTS GlutaMAX Supplement (Cat. No. A4737001) have an intended use statement of “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals”. GlutaMAX Supplement (Cat. No. 35050061) has an intended use statement of “For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals”. The catalog number to use will depend on the specific application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

GlutaMAX Supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture medium. Please go here to read more about the properties of GlutaMAX supplement (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.