这些快速 Western 印迹试剂盒需要的手动操作时间极短,并且得到的结果与使用 SuperSignal West Pico 化学发光底物的传统 Western 印迹相当。该试剂盒包含完成使用小鼠或兔一抗探测的 Western 印迹所需的所有试剂。与其他 Western 印迹法一样,所含的 SuperSignal West Pico 底物产生信号,该信号可通过摄影或其他成像方法进行检测。开发的印迹可以重复暴露,以及被剥离一抗并重新探测。
Pierce 快速 Western 印迹试剂盒是基于试剂的系统,可提供优化的试剂,用于进行 Western 印迹分析中的封闭、抗体稀释和检测。快速 Western 印迹试剂盒方案是一种用于快速、高效地生成 Western 印迹的方法,无需购买其他常用快速 Western 印迹系统所需的昂贵仪器和耗材。
足够用于:用小鼠一抗探测的 5 个微型印迹 • 快速 Western 抗体稀释液,100 mL • 快速 Western 10X 洗涤缓冲液,50 mL • 快速 Western 小鼠优化的 HRP 试剂,Pico,5 mL • SuperSignal West Pico 鲁米诺/增强剂溶液,25 mL • SuperSignal West 稳定过氧化物溶液,25 mL
在 4°C 下储存试剂盒。
常见问题解答 (FAQ)
How can I increase the sensitivity of my Western blot?
Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.