Pierce™ Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal™ West Dura(小鼠)
Pierce™ Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal™ West Dura(小鼠)
Thermo Scientific™

Pierce™ Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal™ West Dura(小鼠)

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid,了解更多信息
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货号数量
35070400 ml
货号 35070
价格(CNY)
7,499.00
Each
添加至购物车
数量:
400 ml
请求批量或定制报价
价格(CNY)
7,499.00
Each
添加至购物车
The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Rabbit
仅供科研使用。不可用于诊断程序。
规格
偶联物HRP
描述Pierce Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal West Dura 底物(小鼠)
反应性小鼠
灵敏度飞克级
足够用于10次 Western 印迹(800 sq. cm 膜)
检测方法化学发光
适用于(应用)蛋白质免疫印迹
形式液体
膜兼容性硝酸纤维素、PVDF
产品线Pierce™、SuperSignal™
产品类型Western 印迹试剂盒
数量400 ml
基质辣根过氧化物酶
Unit SizeEach
内容与储存
足够用于:使用小鼠一抗进行10次小型印迹探查
• Fast Western 抗体稀释液,200 mL
• Fast Western 10X 洗涤缓冲液,100 mL
• Fast Western 小鼠优化 HRP 试剂,Dura,10 mL
• SuperSignal West Dura 鲁米诺/增强剂溶液,50 mL
• SuperSignal West 稳定过氧化物溶液,50 mL

将试剂盒储存于 4°C。

常见问题解答 (FAQ)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.