Pierce™ Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal™ West Femto(小鼠)
Thermo Scientific™
Pierce™ Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal™ West Femto(小鼠)
The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level了解更多信息
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货号
数量
35080
400 ml
货号 35080
价格(CNY)
7,962.00
Each
添加至购物车
数量:
400 ml
请求批量或定制报价
价格(CNY)
7,962.00
Each
添加至购物车
The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.
Features of this kit:
• Fast—femtogram levels of detection in about 1 hour • Convenient—no expensive hardware or vacuum required; no clogging issues • Simple—optimized protocol makes Western blot analysis easier than ever • Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody
The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a mouse primary antibody.
描述Pierce Fast Western Blot 蛋白免疫印迹试剂盒、SuperSignal West Femto 底物(小鼠)
反应性小鼠
灵敏度中低飞克
足够用于10次 Western 印迹(800 sq. cm 膜)
检测方法化学发光
适用于(应用)蛋白质免疫印迹
形式液体
膜兼容性硝酸纤维素、PVDF
产品线Pierce™、SuperSignal™
产品类型Western 印迹试剂盒
数量400 ml
基质辣根过氧化物酶
Unit SizeEach
内容与储存
足够用于:使用小鼠一抗进行10次小型印迹探查 • Fast Western 抗体稀释液,200 mL • Fast Western 10X 洗涤缓冲液,100 mL • Fast Western 小鼠优化 HRP 试剂,Femto,10 mL • SuperSignal West Femto 鲁米诺/增强剂溶液,50 mL • SuperSignal West 稳定过氧化物溶液,50 mL
将试剂盒储存于 4°C。
常见问题解答 (FAQ)
How can I increase the sensitivity of my Western blot?
Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.