Neutral sphingomyelinase inhibitor scyphostatin prevents and ceramide mimics mechanotransduction in vascular endothelium.
AuthorsCzarny M, Schnitzer JE,
JournalAm J Physiol Heart Circ Physiol
PubMed ID15142848
Recently, we showed that neutral sphingomyelinase (N-SMase) is concentrated at the endothelial cell surface in caveolae and is activated to produce ceramide in an acute and transient manner by increase in flow rate and pressure in rat lung vasculature (Czarny M, Liu J, Oh P, and Schnitzer JE, J Biol ... More
The conserved metalloprotease invadolysin localizes to the surface of lipid droplets.
AuthorsCobbe N, Marshall KM, Gururaja Rao S, Chang CW, Di Cara F, Duca E, Vass S, Kassan A, Heck MM,
JournalJ Cell Sci
PubMed ID19706689
'Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent ... More
Identification of a structural determinant necessary for the localization and function of estrogen receptor alpha at the plasma membrane.
AuthorsRazandi M, Alton G, Pedram A, Ghonshani S, Webb P, Levin ER,
JournalMol Cell Biol
PubMed ID12588983
'Estrogen receptors (ER) have been localized to the cell plasma membrane (PM), where signal transduction mediates some estradiol (E2) actions. However, the precise structural features of ER that result in membrane localization have not been determined. We obtained a partial tryptic peptide/mass spectrometry analysis of membrane mouse ERalpha protein. Based ... More