JAM-A is both essential and inhibitory to development of hepatic polarity in WIF-B cells.
AuthorsBraiterman LT, Heffernan S, Nyasae L, Johns D, See AP, Yutzy R, McNickle A, Herman M, Sharma A, Naik UP, Hubbard AL
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID18096610
'Junctional adhesion molecule (JAM) is involved in tight junction (TJ) formation in epithelial cells. Three JAMs (A, B, and C) are expressed in rat hepatocytes, but only rat JAM-A is present in polarized WIF-B cells, a rat-human hepatic line. We used knockdown (KD) and overexpression in WIF-B cells to determine ... More
Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.
AuthorsIvanov AI, Nusrat A, Parkos CA,
JournalMol Biol Cell
PubMed ID14528017
'The adherens junction (AJ) and tight junction (TJ) are key regulators of epithelial polarity and barrier function. Loss of epithelial phenotype is accompanied by endocytosis of AJs and TJs via unknown mechanisms. Using a model of calcium depletion, we defined the pathway of internalization of AJ and TJ proteins (E-cadherin, ... More
rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9.
AuthorsMok KW, Mruk DD, Cheng CY,
Journal
PubMed ID25217631
'Mammalian target of rapamycin complex 1 (mTORC1) is an emerging regulator of blood-tissue barriers that utilizes ribosomal protein S6 (rpS6) as the downstream signaling molecule. To explore the role of rpS6 in blood-testis barrier (BTB) function, a constitutively active quadruple rpS6 phosphomimetic mutant was constructed in mammalian expression vector and ... More
Matrigel improves functional properties of primary human salivary gland cells.
AuthorsMaria OM, Zeitouni A, Gologan O, Tran SD
JournalTissue Eng Part A
PubMed ID21189069
'Currently, there is no effective treatment available to patients with irreversible loss of functional salivary acini caused by Sjogren''s syndrome or after radiotherapy for head and neck cancer. A tissue-engineered artificial salivary gland would help these patients. The graft cells for this device must establish tight junctions in addition to ... More
Conditional forebrain inactivation of nicastrin causes progressive memory impairment and age-related neurodegeneration.
AuthorsTabuchi K, Chen G, Südhof TC, Shen J,
JournalJ Neurosci
PubMed ID19494151
'Loss of presenilin function in adult mouse brains causes memory loss and age-related neurodegeneration. Since presenilin possesses gamma-secretase-dependent and -independent activities, it remains unknown which activity is required for presenilin-dependent memory formation and neuronal survival. To address this question, we generated postnatal forebrain-specific nicastrin conditional knock-out (cKO) mice, in which ... More
Differential effects of flavonoids on barrier integrity in human intestinal caco-2 cells.
AuthorsNoda S, Tanabe S, Suzuki T
JournalJ Agric Food Chem
PubMed ID22506771
'Flavonoids, present in fruits, vegetables, and teas, provide beneficial effects for our health. We investigated the effect of a number of flavonoids on tight junction (TJ) barrier integrity in human intestinal Caco-2 cells. Transepithelial electrical resistance (TER; a TJ integrity marker) across cell monolayers was measured in cells incubated with ... More
The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex.
AuthorsMertens AE, Rygiel TP, Olivo C, van der Kammen R, Collard JG,
JournalJ Cell Biol
PubMed ID16186252
'The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. Similar to wild-type (WT) ... More
Junctional adhesion molecule 1 regulates epithelial cell morphology through effects on beta1 integrins and Rap1 activity.
AuthorsMandell KJ, Babbin BA, Nusrat A, Parkos CA,
JournalJ Biol Chem
PubMed ID15677455
'Epithelial tight junctions form a selectively permeable barrier to ions and small molecules. Junctional adhesion molecule 1 (JAM1/JAM-A/F11R) is a tight junction-associated transmembrane protein that has been shown to participate in the regulation of epithelial barrier function. In a recent study, we presented evidence suggesting that JAM1 homodimer formation is ... More
Distribution of tight junction proteins in adult human salivary glands.
AuthorsMaria OM, Kim JW, Gerstenhaber JA, Baum BJ, Tran SD
JournalJ Histochem Cytochem
PubMed ID18765838
'Tight junctions (TJs) are an essential structure of fluid-secreting cells, such as those in salivary glands. Three major families of integral membrane proteins have been identified as components of the TJ: claudins, occludin, and junctional adhesion molecules (JAMs), plus the cytosolic protein zonula occludens (ZO). We have been working to ... More
Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells.
AuthorsYu AS, McCarthy KM, Francis SA, McCormack JM, Lai J, Rogers RA, Lynch RD, Schneeberger EE,
JournalAm J Physiol Cell Physiol
PubMed ID15689410
The function of occludin (Occ) in the tight junction is undefined. To gain insight into its role in epithelial cell biology, occludin levels in Madin-Darby canine kidney II cells were suppressed by stably expressing short interfering RNA. Suppression of occludin was associated with a decrease in claudins-1 and -7 and ... More
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
AuthorsHaarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
JournalPLoS One
PubMed ID21060661
An inducible release of soluble junctional adhesion molecule-A (sJAM-A) under pro-inflammatory conditions was described in cultured non-CNS endothelial cells (EC) and increased sJAM-A serum levels were found to indicate inflammation in non-CNS vascular beds. Here we studied the regulation of JAM-A expression in cultured brain EC and evaluated sJAM-A as ... More
Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells.
AuthorsYan HH, Mruk DD, Lee WM, Cheng CY,
JournalFASEB J
PubMed ID18192323
During spermatogenesis in the mammalian testis, preleptotene/leptotene spermatocytes differentiate from type B spermatogonia and traverse the blood-testis barrier (BTB) at stage VIII of the seminiferous epithelial cycle for further development. This timely movement of germ cells involves extensive junction restructuring at the BTB. Previous studies have shown that these events ... More
Breast cancer cell migration is regulated through junctional adhesion molecule-A-mediated activation of Rap1 GTPase.
AuthorsMcSherry EA, Brennan K, Hudson L, Hill AD, Hopkins AM,
JournalBreast Cancer Res
PubMed ID21429211
The adhesion protein junctional adhesion molecule-A (JAM-A) regulates epithelial cell morphology and migration, and its over-expression has recently been linked with increased risk of metastasis in breast cancer patients. As cell migration is an early requirement for tumor metastasis, we sought to identify the JAM-A signalling events regulating migration in ... More
Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1.
AuthorsFang X, Neyrinck AP, Matthay MA, Lee JW,
JournalJ Biol Chem
PubMed ID20554518
Acute lung injury is characterized by injury to the lung epithelium that leads to impaired resolution of pulmonary edema and also facilitates accumulation of protein-rich edema fluid and inflammatory cells in the distal airspaces of the lung. Recent in vivo and in vitro studies suggest that mesenchymal stem cells (MSC) ... More
Regulation of the blood-testis barrier by coxsackievirus and adenovirus receptor.
AuthorsSu L, Mruk DD, Cheng CY,
JournalAm J Physiol Cell Physiol
PubMed ID22875787
The blood-testis barrier (BTB) divides the seminiferous epithelium into the basal and the adluminal compartment. It restricts paracellular diffusion of molecules between Sertoli cells, confers cell polarity, and creates a unique microenvironment in the adluminal compartment for spermatid development. However, it undergoes restructuring during the epithelial cycle so that preleptotene ... More