Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification.
AuthorsBjörnson A, Moses J, Ingemansson A, Haraldsson B, Sörensson J,
JournalAm J Physiol Renal Physiol
PubMed ID15585670
This article describes the possible role of the endothelial cell-surface coat, containing proteoglycans (PGs) with connected glycosaminoglycans (GAGs), in maintaining glomerular permselectivity. Primary human glomerular endothelial cells (HGEC) in culture were treated with the nephrosis-inducing agent puromycin aminonucleoside (PAN). Analysis was made by TaqMan real-time PCR, Western blot analysis, and ... More
Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.
AuthorsKalia M, Chandra V, Rahman SA, Sehgal D, Jameel S
JournalJ Virol
PubMed ID19812150
'The hepatitis E virus (HEV), a nonenveloped RNA virus, is the causative agent of hepatitis E. The mode by which HEV attaches to and enters into target cells for productive infection remains unidentified. Open reading frame 2 (ORF2) of HEV encodes its major capsid protein, pORF2, which is likely to ... More