Differential expression of claudin tight junction proteins in the human cortical nephron.
AuthorsKirk A, Campbell S, Bass P, Mason J, Collins J
JournalNephrol Dial Transplant
PubMed ID20124215
In renal tubules, paracellular permeability is tightly controlled to facilitate solute absorption and urinary concentration and is regulated by tight junctions, which incorporate claudin proteins. There is very limited information confirming the localization of these proteins in the human renal cortex. Most data is inferred from mouse, bovine and rabbit ... More
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
AuthorsCollins JE, Kirk A, Campbell SK, Mason J, Wilson SJ,
JournalMethods Mol Biol
PubMed ID21717371
There are a number of disadvantages with conventional tissue immunohistochemistry for accurate -localisation of claudin proteins. Traditionally, tissue cryopreservation or formaldehyde fixation with wax embedding is utilised prior to sectioning and antibody localisation. Wax embedding gives better morphological preservation than frozen tissue, but the required use of chemical cross-linking fixatives ... More