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Invitrogen

Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody

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23 篇参考文献
查看其他68个ERK1/2抗体

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产品信息

36-8800

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
查看15篇已发表文章 15篇已发表文章

免疫组化 (IHC)

1:500
查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500
-

免疫细胞化学 (ICC/IF)

1:100-1:250
查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Plant, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide corresponding to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533283

产品详细信息

This antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology.

36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.

靶标信息

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ERK-1; ERK-2; erk1 erk2; Erk1; cancer susceptibility protein; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2; unnamed protein product

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基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844

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Entrez Gene ID: (Human) 5594, (Human) 5595, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417

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功能
nucleotide binding phosphotyrosine binding DNA binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity transferase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding transcription factor binding transferase activity, transferring phosphorus-containing groups protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding non-receptor serine/threonine protein kinase
参与通路
MAPK cascade regulation of transcription from RNA polymerase II promoter protein phosphorylation apoptotic process chemotaxis cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission heart development learning or memory insulin receptor signaling pathway organ morphogenesis positive regulation of macrophage chemotaxis positive regulation of peptidyl-threonine phosphorylation neural crest cell development Schwann cell development peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance response to lipopolysaccharide regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation cellular response to amino acid starvation response to nicotine intracellular signal transduction ERBB signaling pathway ERBB2-ERBB3 signaling pathway outer ear morphogenesis myelination response to exogenous dsRNA positive regulation of cholesterol biosynthetic process negative regulation of cell differentiation insulin-like growth factor receptor signaling pathway thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade regulation of cytoskeleton organization Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway ERK1 and ERK2 cascade response to epidermal growth factor cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport autophagosome assembly negative regulation of T cell mediated immune response to tumor cell transcription, DNA-templated regulation of gene expression negative regulation of autophagy positive regulation of autophagy phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity cellular response to nutrient levels negative regulation of proteasomal ubiquitin-dependent protein catabolic process peptidyl-tyrosine autophosphorylation TORC1 signaling glucose homeostasis signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus negative regulation of cholesterol efflux xenophagy negative regulation of TORC1 signaling positive regulation of xenophagy MAPK import into nucleus cell cycle response to toxic substance peptidyl-serine phosphorylation response to estrogen positive regulation of translation regulation of sequence-specific DNA binding transcription factor activity cellular response to organic substance cellular response to granulocyte macrophage colony-stimulating factor stimulus positive regulation of cell proliferation positive regulation of cell migration positive regulation of transcription, DNA-templated positive regulation of protein phosphorylation protein complex assembly DNA damage induced protein phosphorylation arachidonic acid metabolic process positive regulation of histone phosphorylation positive regulation of histone acetylation negative regulation of apolipoprotein binding
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