Previously, using an animal model of syndrome X, the obese Zucker rat (OZR), we documented impaired endothelium-dependent vasodilation. The aim of this study was to determine whether reduced expression or altered posttranslational regulation of endothelial nitric oxide synthase (eNOS) underlies the vascular dysfunction in OZR rats. There was no significant ... More
Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.
Authors Fulton David; Fontana Jason; Sowa Grzegorz; Gratton Jean-Philippe; Lin Michelle; Li Kai-Xun; Michell Belinda; Kemp Bruce E; Rodman David; Sessa William C;
JournalJ Biol Chem
PubMed ID11729179
The subcellular localization of endothelial nitric-oxide synthase (eNOS) is critical for optimal coupling of extracellular stimulation to nitric oxide production. Because eNOS is activated by Akt-dependent phosphorylation to produce nitric oxide (NO), we determined the subcellular distribution of eNOS phosphorylated on serine 1179 using a variety of methodologies. Based on ... More
AuthorsNath AK, Enciso J, Kuniyasu M, Hao XY, Madri JA, Pinter E
JournalDevelopment
PubMed ID15128676
Nitric oxide (NO) has been demonstrated to mediate events during ovulation, pregnancy, blastocyst invasion and preimplantation embryogenesis. However, less is known about the role of NO during postimplantation development. Therefore, in this study, we explored the effects of NO during vascular development of the murine yolk sac, which begins shortly ... More